Expanding the list of sequence-agnostic enzymes for chromatin conformation capture assays with S1 nuclease.
Journal
Epigenetics & chromatin
ISSN: 1756-8935
Titre abrégé: Epigenetics Chromatin
Pays: England
ID NLM: 101471619
Informations de publication
Date de publication:
11 Dec 2023
11 Dec 2023
Historique:
received:
23
06
2023
accepted:
24
11
2023
medline:
11
12
2023
pubmed:
11
12
2023
entrez:
10
12
2023
Statut:
epublish
Résumé
This study presents a novel approach for mapping global chromatin interactions using S1 nuclease, a sequence-agnostic enzyme. We develop and outline a protocol that leverages S1 nuclease's ability to effectively introduce breaks into both open and closed chromatin regions, allowing for comprehensive profiling of chromatin properties. Our S1 Hi-C method enables the preparation of high-quality Hi-C libraries, marking a significant advancement over previously established DNase I Hi-C protocols. Moreover, S1 nuclease's capability to fragment chromatin to mono-nucleosomes suggests the potential for mapping the three-dimensional organization of the genome at high resolution. This methodology holds promise for an improved understanding of chromatin state-dependent activities and may facilitate the development of new genomic methods.
Identifiants
pubmed: 38072950
doi: 10.1186/s13072-023-00524-4
pii: 10.1186/s13072-023-00524-4
pmc: PMC10712037
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
48Subventions
Organisme : Russian Science Support Foundation
ID : 22-14-00247
Organisme : Russian Science Support Foundation
ID : 22-14-00247
Informations de copyright
© 2023. The Author(s).
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