Extracellular vesicles and co-isolated endogenous retroviruses from murine cancer cells differentially affect dendritic cells.
antigen presenting cells
endogenous retrovirus
exosomes
extracellular vesicles
tumors
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
11 Dec 2023
11 Dec 2023
Historique:
revised:
31
10
2023
received:
23
01
2023
accepted:
02
11
2023
medline:
11
12
2023
pubmed:
11
12
2023
entrez:
11
12
2023
Statut:
ppublish
Résumé
Cells secrete extracellular vesicles (EVs) and non-vesicular extracellular (nano)particles (NVEPs or ENPs) that may play a role in intercellular communication. Tumor-derived EVs have been proposed to induce immune priming of antigen presenting cells or to be immuno-suppressive agents. We suspect that such disparate functions are due to variable compositions in EV subtypes and ENPs. We aimed to characterize the array of secreted EVs and ENPs of murine tumor cell lines. Unexpectedly, we identified virus-like particles (VLPs) from endogenous murine leukemia virus in preparations of EVs produced by many tumor cells. We established a protocol to separate small EVs from VLPs and ENPs. We compared their protein composition and analyzed their functional interaction with target dendritic cells. ENPs were poorly captured and did not affect dendritic cells. Small EVs specifically induced dendritic cell death. A mixed large/dense EV/VLP preparation was most efficient to induce dendritic cell maturation and antigen presentation. Our results call for systematic re-evaluation of the respective proportions and functions of non-viral EVs and VLPs produced by murine tumors and their contribution to tumor progression.
Identifiants
pubmed: 38073509
doi: 10.15252/embj.2023113590
pmc: PMC10711651
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e113590Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : CC2088
Pays : United Kingdom
Organisme : Wellcome Trust
ID : CC2088
Pays : United Kingdom
Informations de copyright
© 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
Références
Acc Chem Res. 2018 Sep 18;51(9):2305-2313
pubmed: 30156826
Science. 1948 Jun 18;107(2790):648-9
pubmed: 17735505
EMBO J. 2021 Apr 15;40(8):e105492
pubmed: 33709510
Nat Rev Cancer. 2012 Dec;12(12):860-75
pubmed: 23151605
Gastroenterology. 2021 Feb;160(3):958-961.e3
pubmed: 33022277
Proteomics. 2007 Aug;7(15):2553-6
pubmed: 17610305
Mol Cancer. 2019 Mar 30;18(1):59
pubmed: 30925927
Mol Cell Proteomics. 2014 Sep;13(9):2513-26
pubmed: 24942700
Cell Metab. 2008 Sep;8(3):237-48
pubmed: 18762024
Cell Mol Life Sci. 2008 Nov;65(21):3383-98
pubmed: 18818872
Cancer Res. 2012 Oct 1;72(19):4920-30
pubmed: 22865453
Science. 2020 May 22;368(6493):897-901
pubmed: 32381591
Nat Rev Immunol. 2014 Mar;14(3):195-208
pubmed: 24566916
J Extracell Vesicles. 2020 Dec;10(2):e12050
pubmed: 33391636
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10592-7
pubmed: 12947040
J Am Soc Mass Spectrom. 2016 Nov;27(11):1719-1727
pubmed: 27572102
Proteomics. 2011 Sep;11(17):3572-7
pubmed: 21751374
Nature. 1998 Mar 19;392(6673):245-52
pubmed: 9521319
J Cell Biol. 2006 Mar 13;172(6):923-35
pubmed: 16533950
Cell Rep. 2019 Apr 16;27(3):940-954.e6
pubmed: 30956133
Blood. 2004 Nov 15;104(10):3257-66
pubmed: 15284116
Nat Med. 2001 Mar;7(3):297-303
pubmed: 11231627
Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11751-E11760
pubmed: 30478053
Cancer Res. 2008 Feb 15;68(4):1228-35
pubmed: 18281500
Cancer Res. 1988 Sep 1;48(17):4954-8
pubmed: 2842042
Mol Biol Cell. 2001 Nov;12(11):3601-17
pubmed: 11694592
J Extracell Vesicles. 2019 Jul 03;8(1):1628592
pubmed: 31303981
Nature. 2012 Nov 29;491(7426):774-8
pubmed: 23103862
Nat Protoc. 2007;2(10):2480-91
pubmed: 17947990
Cancer Immunol Res. 2018 Nov;6(11):1292-1300
pubmed: 30143537
Nat Cell Biol. 2018 Mar;20(3):332-343
pubmed: 29459780
Oncogene. 2008 Oct 6;27(45):5904-12
pubmed: 18836471
Philos Trans R Soc Lond B Biol Sci. 2018 Jan 5;373(1737):
pubmed: 29158309
Cell. 2019 Apr 4;177(2):428-445.e18
pubmed: 30951670
Nat Methods. 2017 Feb 28;14(3):228-232
pubmed: 28245209
Dev Cell. 2020 Jan 6;52(1):104-117.e5
pubmed: 31866204
Nat Commun. 2021 Jul 19;12(1):4389
pubmed: 34282141
Front Immunol. 2012 Nov 07;3:331
pubmed: 23162549
Curr Protoc Cell Biol. 2006 Apr;Chapter 3:Unit 3.22
pubmed: 18228490
Nat Immunol. 2000 Jul;1(1):54-8
pubmed: 10881175
Immunology. 2004 Jun;112(2):211-8
pubmed: 15147564
J Virol. 2008 Oct;82(19):9329-36
pubmed: 18632860
Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):9155-61
pubmed: 27432966
Virology. 1988 Jan;162(1):1-11
pubmed: 2447699
J Immunol Methods. 2008 Sep 30;338(1-2):21-30
pubmed: 18675270
Front Microbiol. 2017 Dec 12;8:2489
pubmed: 29312197
EMBO J. 2023 Dec 11;42(24):e113590
pubmed: 38073509
Biochem Pharmacol. 2009 Feb 1;77(3):375-83
pubmed: 19014912
Front Immunol. 2018 Jul 03;9:1534
pubmed: 30018618
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6263-7
pubmed: 83652
Blood. 2001 Sep 1;98(5):1512-23
pubmed: 11520802
Front Immunol. 2018 Jun 28;9:1486
pubmed: 30002658
Nat Commun. 2011 Feb 01;2:180
pubmed: 21285958
Nat Rev Immunol. 2009 Aug;9(8):581-93
pubmed: 19498381
J Extracell Vesicles. 2018 Nov 23;7(1):1535750
pubmed: 30637094
Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7
pubmed: 27141961
EMBO J. 2017 Oct 16;36(20):3012-3028
pubmed: 28923825
Mol Biol Cell. 1999 Nov;10(11):3771-86
pubmed: 10564270
J Extracell Vesicles. 2018 Mar 04;7(1):1442985
pubmed: 29535851
Cell. 2020 Aug 20;182(4):1044-1061.e18
pubmed: 32795414
Nucleic Acids Res. 2022 Jan 7;50(D1):D543-D552
pubmed: 34723319
Methods Mol Biol. 2013;960:327-350
pubmed: 23329497
Blood Cells Mol Dis. 2005 Sep-Oct;35(2):81-8
pubmed: 15982908
Semin Cell Dev Biol. 2015 Apr;40:72-81
pubmed: 25724562
Nat Cell Biol. 2021 Dec;23(12):1240-1254
pubmed: 34887515
Front Immunol. 2018 Apr 30;9:738
pubmed: 29760691
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4188-92
pubmed: 6330748
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4480-4
pubmed: 8506289
Science. 1995 Mar 3;267(5202):1275-6
pubmed: 7871422
Cancer. 1952 Mar;5(2):382-402
pubmed: 14905426
Nat Immunol. 2013 Oct;14(10):1014-22
pubmed: 24048123
J Cell Biol. 1999 Nov 1;147(3):599-610
pubmed: 10545503
J Immunol. 2001 Jun 15;166(12):7309-18
pubmed: 11390481
Biologicals. 2003 Dec;31(4):251-60
pubmed: 14624795
Philos Trans R Soc Lond B Biol Sci. 2017 Oct 19;372(1732):
pubmed: 28893944