Is Autophagy a Prerequisite for Steroidogenesis in Leydig Cells?
Autophagy
Autophagy inhibitor
Leydig cells
Microtubule associated protein light chain 3 (LC3)
Steroidogenesis
Journal
Development & reproduction
ISSN: 2465-9525
Titre abrégé: Dev Reprod
Pays: Korea (South)
ID NLM: 101178352
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
03
07
2023
revised:
30
08
2023
accepted:
16
09
2023
medline:
11
12
2023
pubmed:
11
12
2023
entrez:
11
12
2023
Statut:
ppublish
Résumé
We investigated the involvement of autophagy with steroidogenesis in testicular Leydig cells. Human chorionic gonadotropin (hCG)-stimulated T production in Leydig cells was not remarkably altered in the presence of an autophagy inhibitor 3-methyladenine (3-MA). Although pretreatment with 3-MA demonstrated a tendency to decrease hCG-induced T production, the differences were significant only at a higher time point of 24 h following hCG. Microtubule associated protein light chain 3 (LC3)-II was detectable in the control cells in all the experiments. The hCG-induced increase in steroidogenic acute regulatory protein (StAR) and cytochrome P450 side chain cleave (P450scc) protein levels were not significantly altered by 3-MA. Leydig cells isolated from immature rat testes 12 h following hCG treatment showed relatively increased levels of LC3-II protein compared to the control group. Furthermore, LC3-II levels shown in these cells reached almost the identical to those from normal adult testes. However, LC3-II protein levels were almost comparable or even slightly lower than the controls at 48 h following hCG. Expression of StAR and P450scc was upregulated at both 12 and 48 h after hCG. We also used MA-10 cells, the mouse Leydig cell line, in this experiment. When dibutyryl cyclic-AMP was treated with MA-10 cells, P4 levels were significantly increased in the cell culture medium. However, P4 levels tended to decrease in the presence of 3-MA, but the difference was not statistically significant. This was consistent with the results of the rat Leydig cell experiments. Together, we believe that although autophagy participates in steroidogenesis and enhances steroidogenic efficacy of Leydig cells, it may not be a decisive cellular process for steroidogenesis, specifically in the mature Leydig cells.
Identifiants
pubmed: 38074457
doi: 10.12717/DR.2023.27.3.149
pii: dr-27-3-149
pmc: PMC10703103
doi:
Types de publication
Journal Article
Langues
eng
Pagination
149-157Informations de copyright
Copyright © 2023 The Korean Society of Developmental Biology.
Déclaration de conflit d'intérêts
The authors declare no potential conflict of interest.
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