Mitochondrial temperature homeostasis resists external metabolic stresses.
D. melanogaster
OXPHOS
biochemistry
bioenergetics
cell biology
chemical biology
human
mitochondria
mouse
organelle
temperature
thermogenesis
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
11 Dec 2023
11 Dec 2023
Historique:
medline:
11
12
2023
pubmed:
11
12
2023
entrez:
11
12
2023
Statut:
epublish
Résumé
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric fluorescent temperature indicator targeted to mitochondria, the temperature of active mitochondria in four mammalian and one insect cell line was estimated to be up to 15°C above that of the external environment to which the cells were exposed. High mitochondrial temperature was maintained in the face of a variety of metabolic stresses, including substrate starvation or modification, decreased ATP demand due to inhibition of cytosolic protein synthesis, inhibition of the mitochondrial adenine nucleotide transporter and, if an auxiliary pathway for electron transfer was available via the alternative oxidase, even respiratory poisons acting downstream of oxidative phosphorylation (OXPHOS) complex I. We propose that the high temperature of active mitochondria is an inescapable consequence of the biochemistry of OXPHOS and is homeostatically maintained as a primary feature of mitochondrial metabolism.
Identifiants
pubmed: 38079477
doi: 10.7554/eLife.89232
pii: 89232
pmc: PMC10712956
doi:
pii:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Academy of Finland
ID : 324730
Organisme : Basic Science Research Institute Fund
ID : 2021R1A6A1A10042944
Organisme : Sigrid Juséliuksen Säätiö
ID : 3122800849
Organisme : Japan Science and Technology Agency
ID : 10.52926/jpmjcr15n3
Organisme : National Research Foundation of Korea
ID : 2023R1A2C300453411
Organisme : Academy of Finland
ID : 322732
Organisme : Academy of Finland
ID : 328969
Informations de copyright
© 2023, Terzioglu et al.
Déclaration de conflit d'intérêts
MT, KV, TM, TI, TS, PB, PR, YC, TN, HJ No competing interests declared
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