Fusobacterium nucleatum exacerbates the progression of pulpitis by regulating the STING-dependent pathway.
Fusobacterium nucleatum
STING
autophagy
mitochondrial DNA
pulpitis
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
revised:
30
10
2023
received:
14
08
2023
accepted:
22
11
2023
medline:
12
12
2023
pubmed:
12
12
2023
entrez:
12
12
2023
Statut:
ppublish
Résumé
Bacterial infection is the main cause of pulpitis. However, whether a dominant bacteria can promote the progression of pulpitis and its underlying mechanism remains unclear. We provided a comprehensive assessment of the microbiota alteration in pulpitis using 16S rRNA sequencing. Fusobacterium nucleatum was the most enriched in pulpitis and played a pathogenic role accelerating pulpitis progression in rat pulpitis model. After odontoblast-like cells cocultured with F. nucleatum, the stimulator of interferon genes (STING) pathway and autophagy were activation. There was a float of STING expression during F. nucleatum stimulation. STING was degraded by autophagy at the early stage. At the late stage, F. nucleatum stimulated mitochondrial Reactive Oxygen Species (ROS) production, mitochondrial dysfunction and then mtDNA escape into cytosol. mtDNA, which escaped into cytosol, caused more cytosolic mtDNA binds to cyclic GMP-AMP synthase (cGAS). The release of IFN-β was dramatically reduced when mtDNA-cGAS-STING pathway inhibited. STING
Identifiants
pubmed: 38085169
doi: 10.1096/fj.202301648R
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23357Subventions
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 82170941
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 81974148
Informations de copyright
© 2023 Federation of American Societies for Experimental Biology.
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