Metagenomic and Molecular Detection of Novel Fecal Viruses in Free-Ranging Agile Wallabies.
Adenoviruses
Feces
Free-ranging agile wallaby
Herpesvirus
Metagenomics
PCR
Parvoviruses
Picobirnaviruses
Polyomaviruses
Viruses
Journal
EcoHealth
ISSN: 1612-9210
Titre abrégé: Ecohealth
Pays: United States
ID NLM: 101222144
Informations de publication
Date de publication:
13 Dec 2023
13 Dec 2023
Historique:
received:
16
12
2022
accepted:
26
10
2023
medline:
13
12
2023
pubmed:
13
12
2023
entrez:
13
12
2023
Statut:
aheadofprint
Résumé
The agile wallaby (Notamacropus agilis) is one of the most abundant marsupial species in northern Queensland and a competent host for the zoonotic Ross River virus. Despite their increased proximity and interactions with humans, little is known about the viruses carried by these animals, and whether any are of conservation or zoonotic importance. Metagenomics and molecular techniques were used in a complementary manner to identify and characterize novel viruses in the fecal samples of free-ranging agile wallabies. We detected a variety of novel marsupial-related viral species including agile wallaby atadenovirus 1, agile wallaby chaphamaparvovirus 1-2, agile wallaby polyomavirus 1-2, agile wallaby associated picobirnavirus 1-9, and a known macropod gammaherpesvirus 3. Phylogenetic analyses indicate that most of these novel viruses would have co-evolved with their hosts (agile wallabies). Additionally, non-marsupial viruses that infect bacteria (phages), plants, insects, and other eukaryotes were identified. This study highlighted the utility of non-invasive sampling as well as the integration of broad-based molecular assays (consensus PCR and next generation sequencing) for monitoring the emergence of potential pathogenic viruses in wildlife species. Furthermore, the novel marsupial viruses identified in this study will enrich the diversity of knowledge about marsupial viruses, and may be useful for developing diagnostics and vaccines.
Identifiants
pubmed: 38091182
doi: 10.1007/s10393-023-01659-2
pii: 10.1007/s10393-023-01659-2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023. EcoHealth Alliance.
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