Greenness assessment and validation of HPLC method for simultaneous determination of Resveratrol and Vitamin E in dietary supplements.
Journal
Journal of AOAC International
ISSN: 1944-7922
Titre abrégé: J AOAC Int
Pays: England
ID NLM: 9215446
Informations de publication
Date de publication:
13 Dec 2023
13 Dec 2023
Historique:
received:
03
07
2023
revised:
18
11
2023
accepted:
20
11
2023
medline:
14
12
2023
pubmed:
14
12
2023
entrez:
13
12
2023
Statut:
aheadofprint
Résumé
There is an increasing interest in the use of a combination of trans-resveratrol and vitamin E in dietary supplements. Determination of the content of both components is essential for confirmation of the quality of the product. To establish the applicability and ensure the greenness of the previously developed high-throughput HPLC/UV method for the simultaneous determination of trans-resveratrol and alpha-tocopherol acetate (vitamin E) in dietary supplements. Separation was performed on RP C8 Select B chromatographic column, using acetonitrile and water in the mobile phase, with gradient elution. Full method validation was performed in accordance with ICH Q2(R1). The greenness of the method was assessed using the analytical eco-scale (AES) methodology and the analytical greenness metric (AGREE). The method is selective, linear, precise and accurate over defined concentration ranges (185-369 µg/mL of trans-resveratrol and 37-75 µg/mL of alpha-tocopherol acetate), and has a suitable sensitivity (limits of detection and quantification are 7.7 µg/mL and 23.3 µg/mL for resveratrol and 2.6 µg/mL and 7.8 µg/mL for tocopherol acetate, respectively). The obtained analytical eco-scale score of 77 and the pale green AGREE pictogram with an overall score of 0.61 confirm the method's greenness. The sensitivity and selectivity of the method, its short analysis time (7 minutes), the low negative environmental impact, and simple sample preparation make the method readily applicable to in-line quality control procedures. A method for simultaneously analysing vitamin E and resveratrol in dietary supplements is presented. The method is rapid, includes a simple sample preparation procedure, and has a low environmental impact.
Sections du résumé
BACKGROUND
BACKGROUND
There is an increasing interest in the use of a combination of trans-resveratrol and vitamin E in dietary supplements. Determination of the content of both components is essential for confirmation of the quality of the product.
OBJECTIVE
OBJECTIVE
To establish the applicability and ensure the greenness of the previously developed high-throughput HPLC/UV method for the simultaneous determination of trans-resveratrol and alpha-tocopherol acetate (vitamin E) in dietary supplements.
METHODS
METHODS
Separation was performed on RP C8 Select B chromatographic column, using acetonitrile and water in the mobile phase, with gradient elution. Full method validation was performed in accordance with ICH Q2(R1). The greenness of the method was assessed using the analytical eco-scale (AES) methodology and the analytical greenness metric (AGREE).
RESULTS
RESULTS
The method is selective, linear, precise and accurate over defined concentration ranges (185-369 µg/mL of trans-resveratrol and 37-75 µg/mL of alpha-tocopherol acetate), and has a suitable sensitivity (limits of detection and quantification are 7.7 µg/mL and 23.3 µg/mL for resveratrol and 2.6 µg/mL and 7.8 µg/mL for tocopherol acetate, respectively). The obtained analytical eco-scale score of 77 and the pale green AGREE pictogram with an overall score of 0.61 confirm the method's greenness.
CONCLUSION
CONCLUSIONS
The sensitivity and selectivity of the method, its short analysis time (7 minutes), the low negative environmental impact, and simple sample preparation make the method readily applicable to in-line quality control procedures.
HIGHLIGHTS
CONCLUSIONS
A method for simultaneously analysing vitamin E and resveratrol in dietary supplements is presented. The method is rapid, includes a simple sample preparation procedure, and has a low environmental impact.
Identifiants
pubmed: 38092031
pii: 7471864
doi: 10.1093/jaoacint/qsad131
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. All rights reserved. For permissions, please email: journals.permissions@oup.com.