Why U matters: detection and functions of pseudouridine modifications in mRNAs.

mRNA modifications mRNA stability pre-mRNA processing pseudouridine splicing translation

Journal

Trends in biochemical sciences
ISSN: 0968-0004
Titre abrégé: Trends Biochem Sci
Pays: England
ID NLM: 7610674

Informations de publication

Date de publication:
14 Dec 2023
Historique:
received: 02 08 2023
revised: 24 10 2023
accepted: 25 10 2023
medline: 15 12 2023
pubmed: 15 12 2023
entrez: 14 12 2023
Statut: aheadofprint

Résumé

The uridine modifications pseudouridine (Ψ), dihydrouridine, and 5-methyluridine are present in eukaryotic mRNAs. Many uridine-modifying enzymes are associated with human disease, underscoring the importance of uncovering the functions of uridine modifications in mRNAs. These modified uridines have chemical properties distinct from those of canonical uridines, which impact RNA structure and RNA-protein interactions. Ψ, the most abundant of these uridine modifications, is present across (pre-)mRNAs. Recent work has shown that many Ψs are present at intermediate to high stoichiometries that are likely conducive to function and at locations that are poised to influence pre-/mRNA processing. Technological innovations and mechanistic investigations are unveiling the functions of uridine modifications in pre-mRNA splicing, translation, and mRNA stability, which are discussed in this review.

Identifiants

pubmed: 38097411
pii: S0968-0004(23)00277-3
doi: 10.1016/j.tibs.2023.10.008
pii:
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no conflicts of interest.

Auteurs

Rebecca Rodell (R)

Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA.

Nicolas Robalin (N)

Department of Chemistry, Stanford University, Stanford, CA 94305, USA.

Nicole M Martinez (NM)

Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA; Sarafan ChEM-H Institute, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: nicolemm@stanford.edu.

Classifications MeSH