Identification, classification, and characterization of alpha and beta subunits of LVP1 protein from the venom gland of four Iranian scorpion species.

Journal

Scientific reports

ISSN: 2045-2322

Titre abrégé: Sci Rep

Pays: England

ID NLM: 101563288

Informations de publication

Date de publication:
14 Dec 2023

Historique:

received: 11 06 2023

accepted: 09 12 2023

medline: 15 12 2023

pubmed: 15 12 2023

entrez: 14 12 2023

Statut: epublish

Résumé

Scorpion venoms contain bioactive peptides and proteins. Some, can be used for pharmaceutical purposes. So, identification of venom proteins matters because, in addition to determining the function of the toxins can also be an excellent guide to developing new drugs. Here, we got transcriptome of venom glands from four Iranian scorpion species, including Hemsicorpius lepturus, Mesobuthus eupeus, Andructunus crassicuada, and Hottentotta saulcyi using cDNA library synthesis and high-throughput transcriptomic analysis of the venom glands. In a comparative way, we identified the cDNA encoding isoforms of subunits (alpha and beta) of BotLVP1/BmLVP1-like protein in the venom gland of three species except for H. lepturus. Characterization and structure determination of the LVP1_like proteins combined with gene map analysis provided evidence of the existence of some isoforms of LVP1_like proteins, encoded by genes with two exons and one intron, which can be classified in CSαβ superfamily in the venom gland of three Iranian scorpion species. According to the high similarity with BotLVP1 and BmLVP1, these proteins could also be potent to mediate cholesterol homeostasis. However, further research is needed to prove it, and this study just may lay the foundation lead to light up this way.

Identifiants

DOI: 10.1038/s41598-023-49556-6 PMID: 38097679

pubmed: 38097679

doi: 10.1038/s41598-023-49556-6

pii: 10.1038/s41598-023-49556-6

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

22277

Subventions

Organisme : Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO)

ID : 960354-020-18-83-2

Organisme : Ahvaz Jundishapur University of medical sciences

ID : TRC9819

Informations de copyright

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