Antibiotic resistance, bacterial transmission and improved prediction of bacterial infection in patients with antibody deficiency.

Journal

JAC-antimicrobial resistance
ISSN: 2632-1823
Titre abrégé: JAC Antimicrob Resist
Pays: England
ID NLM: 101765283

Informations de publication

Date de publication:
Dec 2023
Historique:
received: 04 10 2023
accepted: 29 11 2023
medline: 15 12 2023
pubmed: 15 12 2023
entrez: 15 12 2023
Statut: epublish

Résumé

Antibody-deficient patients are at high risk of respiratory tract infections. Many therefore receive antibiotic prophylaxis and have access to antibiotics for self-administration in the event of breakthrough infections, which may increase antimicrobial resistance (AMR). To understand AMR in the respiratory tract of patients with antibody deficiency. Sputum samples were collected from antibody-deficient patients in a cross-sectional and prospective study; bacteriology culture, 16S rRNA profiling and PCR detecting macrolide resistance genes were performed. Bacterial isolates were identified using MALDI-TOF, antimicrobial susceptibility was determined by disc diffusion and WGS of selected isolates was done using Illumina NextSeq with analysis for resistome and potential cross-transmission. Neutrophil elastase was measured by a ProteaseTag immunoassay. Three hundred and forty-three bacterial isolates from sputum of 43 patients were tested. Macrolide and tetracycline resistance were common (82% and 35% of isolates). Measures to limit antibiotic usage and spread of AMR should be implemented in immunodeficiency clinics. Sputum neutrophil elastase may be a useful marker to guide use of antibiotics for respiratory infection.

Sections du résumé

Background UNASSIGNED
Antibody-deficient patients are at high risk of respiratory tract infections. Many therefore receive antibiotic prophylaxis and have access to antibiotics for self-administration in the event of breakthrough infections, which may increase antimicrobial resistance (AMR).
Objectives UNASSIGNED
To understand AMR in the respiratory tract of patients with antibody deficiency.
Methods UNASSIGNED
Sputum samples were collected from antibody-deficient patients in a cross-sectional and prospective study; bacteriology culture, 16S rRNA profiling and PCR detecting macrolide resistance genes were performed. Bacterial isolates were identified using MALDI-TOF, antimicrobial susceptibility was determined by disc diffusion and WGS of selected isolates was done using Illumina NextSeq with analysis for resistome and potential cross-transmission. Neutrophil elastase was measured by a ProteaseTag immunoassay.
Results UNASSIGNED
Three hundred and forty-three bacterial isolates from sputum of 43 patients were tested. Macrolide and tetracycline resistance were common (82% and 35% of isolates).
Conclusions UNASSIGNED
Measures to limit antibiotic usage and spread of AMR should be implemented in immunodeficiency clinics. Sputum neutrophil elastase may be a useful marker to guide use of antibiotics for respiratory infection.

Identifiants

DOI: 10.1093/jacamr/dlad135 PMID: 38098890 PMC: PMC10720947
pubmed: 38098890
doi: 10.1093/jacamr/dlad135
pii: dlad135
pmc: PMC10720947
doi:

Types de publication

Journal Article

Langues

eng

Pagination

dlad135

Informations de copyright

© The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.

Auteurs

Sylvia Rofael (S)

Centre for Clinical Microbiology, University College London, Royal Free Campus, Pond Street, London, UK.
Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt.

Clara Leboreiro Babe (C)

Centre for Clinical Microbiology, University College London, Royal Free Campus, Pond Street, London, UK.

Mehmet Davrandi (M)

Centre for Clinical Microbiology, University College London, Royal Free Campus, Pond Street, London, UK.

Alexandra L Kondratiuk (AL)

Institute for Immunity and Transplantation, Division of Infection and Immunity, University College London, Pears Building, Rowland Hill Street, London, UK.

Leanne Cleaver (L)

Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, Guy's Campus, King's College London, London, UK.

Naseem Ahmed (N)

Centre for Clinical Microbiology, University College London, Royal Free Campus, Pond Street, London, UK.

Claire Atkinson (C)

Institute for Immunity and Transplantation, Division of Infection and Immunity, University College London, Pears Building, Rowland Hill Street, London, UK.
Cancer Biology and Therapy Research Group, Divisionof Human Sciences, School of Applied Sciences, London South Bank University, London, UK.

Timothy McHugh (T)

Centre for Clinical Microbiology, University College London, Royal Free Campus, Pond Street, London, UK.

David M Lowe (DM)

Institute for Immunity and Transplantation, Division of Infection and Immunity, University College London, Pears Building, Rowland Hill Street, London, UK.
Department of Clinical Immunology, Royal Free London NHS Foundation Trust, Pond Street, London, UK.
ORCID: 0000-0001-6102-2375

Classifications MeSH