Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage.
ICSI
encapsulation
lipidomics
ovine
polyunsaturated fatty acids
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2023
2023
Historique:
received:
31
07
2023
accepted:
14
11
2023
medline:
15
12
2023
pubmed:
15
12
2023
entrez:
15
12
2023
Statut:
epublish
Résumé
Freeze-drying techniques give alternative preservation mammalian spermatozoa without liquid nitrogen. However, most of the work has been conducted in the laboratory mouse, while little information has been gathered on large animals that could also benefit from this kind of storage. This work adapted a technique known as vacuum-drying encapsulation (VDE), originally developed for nucleic acid conservation in anhydrous state, to ram spermatozoa, and compared it to canonical lyophilization (FD), testing long-term storage at room temperature (RT) and 4°C. The results demonstrated better structural stability, namely lipid composition and DNA integrity, in VDE spermatozoa than FD ones, with outcomes at RT storage comparable to 4°C. Likewise, in VDE the embryonic development was higher than in FD samples (12.8% vs. 8.7%,
Identifiants
pubmed: 38098985
doi: 10.3389/fvets.2023.1270266
pmc: PMC10720722
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1270266Informations de copyright
Copyright © 2023 Palazzese, Turri, Anzalone, Saragusty, Bonnet, Colotte, Tuffet, Pizzi, Luciani, Matsukawa, Czernik and Loi.
Déclaration de conflit d'intérêts
JB, MC, and ST were employed by the company Imagene. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.