Individual genotypes from environmental DNA: Fingerprinting snow tracks of three large carnivore species.
eDNA-based population studies
high-throughput STR genotyping by sequencing
individual identification
large carnivores
non-invasive genetics
snow track sampling
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
15 Dec 2023
15 Dec 2023
Historique:
revised:
24
11
2023
received:
28
05
2023
accepted:
30
11
2023
medline:
15
12
2023
pubmed:
15
12
2023
entrez:
15
12
2023
Statut:
aheadofprint
Résumé
Continued advancements in environmental DNA (eDNA) research have made it possible to access intraspecific variation from eDNA samples, opening new opportunities to expand non-invasive genetic studies of wildlife populations. However, the use of eDNA samples for individual genotyping, as typically performed in non-invasive genetics, still remains elusive. We present successful individual genotyping of eDNA obtained from snow tracks of three large carnivores: brown bear (Ursus arctos), European lynx (Lynx lynx) and wolf (Canis lupus). DNA was extracted using a protocol for isolating water eDNA and genotyped using amplicon sequencing of short tandem repeats (STR), and for brown bear a sex marker, on a high-throughput sequencing platform. Individual genotypes were obtained for all species, but genotyping performance differed among samples and species. The proportion of samples genotyped to individuals was higher for brown bear (5/7) and wolf (7/10) than for lynx (4/9), and locus genotyping success was greater for brown bear (0.88). The sex marker was typed in six out of seven brown bear samples. Results for three species show that reliable individual genotyping, including sex identification, is now possible from eDNA in snow tracks, underlining its vast potential to complement the non-invasive genetic methods used for wildlife. To fully leverage the application of snow track eDNA, improved understanding of the ideal species- and site-specific sampling conditions, as well as laboratory methods promoting genotyping success, is needed. This will also inform efforts to retrieve and type nuclear DNA from other eDNA samples, thereby advancing eDNA-based individual and population-level studies.
Identifiants
pubmed: 38099394
doi: 10.1111/1755-0998.13915
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13915Subventions
Organisme : European Commission
ID : LIFE16 NAT/SI/000634
Organisme : European Commission
ID : LIFE18 NAT/IT/000972
Organisme : Javna Agencija za Raziskovalno Dejavnost RS
ID : P1-0184
Organisme : Swiss Federal Office for the Environment (FOEN)
Informations de copyright
© 2023 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
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