Generation of highly pure pluripotent stem cell-derived myogenic progenitor cells and myotubes.
hiPSC
mESC
muscle progenitors
myogenic differentiation
myotubes
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
12 Dec 2023
12 Dec 2023
Historique:
received:
04
01
2023
revised:
14
11
2023
accepted:
15
11
2023
medline:
16
12
2023
pubmed:
16
12
2023
entrez:
15
12
2023
Statut:
aheadofprint
Résumé
Driving efficient and pure skeletal muscle cell differentiation from pluripotent stem cells (PSCs) has been challenging. Here, we report an optimized protocol that generates skeletal muscle progenitor cells with high efficiency and purity in a short period of time. Human induced PSCs (hiPSCs) and murine embryonic stem cells (mESCs) were specified into the mesodermal myogenic fate using distinct and species-specific protocols. We used a specific maturation medium to promote the terminal differentiation of both human and mouse myoblast populations, and generated myotubes associated with a large pool of cell-cycle arrested PAX7
Identifiants
pubmed: 38101399
pii: S2213-6711(23)00451-4
doi: 10.1016/j.stemcr.2023.11.002
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2023. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of interests R.B.A., C.L., J.E.d.L., and F.R. declare no competing interests. D.A., J.M., and B.O. work or have worked for Phenocell.