Rapid characterization of adeno-associated virus (AAV) capsid proteins using microchip ZipChip CE-MS.
Adeno-associated virus
Characterization
Gene therapy
Microchip CE-MS
Post translation modifications
Product quality monitoring
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
15 Dec 2023
15 Dec 2023
Historique:
received:
08
11
2023
accepted:
04
12
2023
revised:
01
12
2023
medline:
16
12
2023
pubmed:
16
12
2023
entrez:
15
12
2023
Statut:
aheadofprint
Résumé
Adeno-associated viruses (AAVs) are viral vectors used as delivery systems for gene therapies. Intact protein characterization of AAV viral capsid proteins (VPs) and their post-translational modifications is critical to ensuring product quality. In this study, microchip-based ZipChip capillary electrophoresis-mass spectrometry (CE-MS) was applied for the rapid characterization of AAV intact VPs, specifically full and empty viral capsids of serotypes AAV6, AAV8 and AAV9, which was accomplished using 5 min of analysis time. Low levels of dimethyl sulfoxide (4%) in the background electrolyte (BGE) improved MS signal quality and component detection. A sensitivity evaluation revealed consistent detection of VP proteoforms when as little as 2.64 × 10
Identifiants
pubmed: 38102410
doi: 10.1007/s00216-023-05097-5
pii: 10.1007/s00216-023-05097-5
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : 908 Devices
ID : NIBRT 908 CRA
Informations de copyright
© 2023. The Author(s).
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