Trypanosome diversity in small mammals in Uganda and the spread of Trypanosoma lewisi to native species.
Aneza
Herpetosoma
Muridae
Ornithotrypanum
Soricidae
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
16 Dec 2023
16 Dec 2023
Historique:
received:
22
08
2023
accepted:
03
11
2023
medline:
16
12
2023
pubmed:
16
12
2023
entrez:
15
12
2023
Statut:
epublish
Résumé
Uganda's diverse small mammalian fauna thrives due to its rich habitat diversity, which hosts a wide range of blood parasites, including trypanosomes, particularly the subgenus Herpetosoma typical for rodent hosts. We screened a total of 711 small mammals from various habitats for trypanosomes, with 253 microscopically examined blood smears and 458 tissue samples tested by nested PCR of the 18S rRNA gene. Of 51 rodent and 12 shrew species tested, microscopic screening reaches 7% overall prevalence (with four rodent species positive out of 15 and none of the shrew species out of four), while nested PCR indicated a prevalence of 13% (17 rodent and five shrew species positive out of 49 and 10, respectively). We identified 27 genotypes representing 11 trypanosome species, of which the majority (24 genotypes/9 species) belong to the Herpetosoma subgenus. Among these, we detected 15 new genotypes and two putative new species, labeled AF24 (found in Lophuromys woosnami) and AF25 (in Graphiurus murinus). Our finding of three new genotypes of the previously detected species AF01 belonging to the subgenus Ornithotrypanum in two Grammomys species and Oenomys hypoxanthus clearly indicates the consistent occurrence of this avian trypanosome in African small mammals. Additionally, in Aethomys hindei, we detected the putative new species of the subgenus Aneza. Within the T. lewisi subclade, we detected eleven genotypes, including six new; however, only the genotype AF05b from Mus and Rattus represents the invasive T. lewisi. Our study has improved our understanding of trypanosome diversity in African small mammals. The detection of T. lewisi in native small mammals expands the range of host species and highlighting the need for a broader approach to the epidemiology of T. lewisi.
Identifiants
pubmed: 38102492
doi: 10.1007/s00436-023-08048-2
pii: 10.1007/s00436-023-08048-2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
54Subventions
Organisme : The Czech Science Foundation
ID : 20-07091J
Organisme : The Czech Science Foundation
ID : 20-07091J
Organisme : The Czech Science Foundation
ID : 20-07091J
Organisme : Czech Science Foundation
ID : 23-06116S
Organisme : Czech Science Foundation
ID : 23-06116S
Organisme : Czech Science Foundation
ID : 23-06116S
Organisme : Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development (ACE IRPM & BTD)
ID : 5799-TZ
Organisme : Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development (ACE IRPM & BTD)
ID : 5799-TZ
Organisme : Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development (ACE IRPM & BTD)
ID : 5799-TZ
Organisme : Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development (ACE IRPM & BTD)
ID : 5799-TZ
Organisme : Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development (ACE IRPM & BTD)
ID : 5799-TZ
Informations de copyright
© 2023. The Author(s).
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