Immunotheranostic target modules for imaging and navigation of UniCAR T-cells to strike FAP-expressing cells and the tumor microenvironment.

3D in vitro models Cancer immunotherapy Fibroblast activation protein (FAP) Immunotheranostic Target Modules (TMs) Tumor microenvironment (TME) UniCAR T-cells

Journal

Journal of experimental & clinical cancer research : CR
ISSN: 1756-9966
Titre abrégé: J Exp Clin Cancer Res
Pays: England
ID NLM: 8308647

Informations de publication

Date de publication:
15 Dec 2023
Historique:
received: 15 08 2023
accepted: 21 11 2023
medline: 16 12 2023
pubmed: 16 12 2023
entrez: 16 12 2023
Statut: epublish

Résumé

Chimeric antigen receptor (CAR) T-cells are a promising approach in cancer immunotherapy, particularly for treating hematologic malignancies. Yet, their effectiveness is limited when tackling solid tumors, where immune cell infiltration and immunosuppressive tumor microenvironments (TME) are major hurdles. Fibroblast activation protein (FAP) is highly expressed on cancer-associated fibroblasts (CAFs) and various tumor cells, playing an important role in tumor growth and immunosuppression. Aiming to modulate the TME with increased clinical safety and effectiveness, we developed novel small and size-extended immunotheranostic UniCAR target modules (TMs) targeting FAP. The specific binding and functionality of the αFAP-scFv TM and the size-extended αFAP-IgG4 TM were assessed using 2D and 3D in vitro models as well as in vivo. Their specific tumor accumulation and diagnostic potential were evaluated using PET studies after functionalization with a chelator and suitable radionuclide. The αFAP-scFv and -IgG4 TMs effectively and specifically redirected UniCAR T-cells using 2D, 3D, and in vivo models. Moreover, a remarkably high and specific accumulation of radiolabeled FAP-targeting TMs at the tumor site of xenograft mouse models was observed. These findings demonstrate that the novel αFAP TMs are promising immunotheranostic tools to foster cancer imaging and treatment, paving the way for a more convenient, individualized, and safer treatment of cancer patients.

Sections du résumé

BACKGROUND BACKGROUND
Chimeric antigen receptor (CAR) T-cells are a promising approach in cancer immunotherapy, particularly for treating hematologic malignancies. Yet, their effectiveness is limited when tackling solid tumors, where immune cell infiltration and immunosuppressive tumor microenvironments (TME) are major hurdles. Fibroblast activation protein (FAP) is highly expressed on cancer-associated fibroblasts (CAFs) and various tumor cells, playing an important role in tumor growth and immunosuppression. Aiming to modulate the TME with increased clinical safety and effectiveness, we developed novel small and size-extended immunotheranostic UniCAR target modules (TMs) targeting FAP.
METHODS METHODS
The specific binding and functionality of the αFAP-scFv TM and the size-extended αFAP-IgG4 TM were assessed using 2D and 3D in vitro models as well as in vivo. Their specific tumor accumulation and diagnostic potential were evaluated using PET studies after functionalization with a chelator and suitable radionuclide.
RESULTS RESULTS
The αFAP-scFv and -IgG4 TMs effectively and specifically redirected UniCAR T-cells using 2D, 3D, and in vivo models. Moreover, a remarkably high and specific accumulation of radiolabeled FAP-targeting TMs at the tumor site of xenograft mouse models was observed.
CONCLUSIONS CONCLUSIONS
These findings demonstrate that the novel αFAP TMs are promising immunotheranostic tools to foster cancer imaging and treatment, paving the way for a more convenient, individualized, and safer treatment of cancer patients.

Identifiants

pubmed: 38102692
doi: 10.1186/s13046-023-02912-w
pii: 10.1186/s13046-023-02912-w
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

341

Subventions

Organisme : Helmholtz Initiative and Networking Fund
ID : InterLabs-0031
Organisme : Bundesministerium für Bildung und Forschung
ID : 03ZU1111LA
Organisme : Bundesministerium für Bildung und Forschung
ID : 03ZU1111LB
Organisme : Bundesministerium für Bildung und Forschung
ID : 03ZU1111LB
Organisme : Deutsche Krebshilfe
ID : Mildred Scheel Early Career Center Dresden P2

Informations de copyright

© 2023. The Author(s).

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Auteurs

Liliana R Loureiro (LR)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany. l.loureiro@hzdr.de.

Lydia Hoffmann (L)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

Christin Neuber (C)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

Luise Rupp (L)

Institute of Immunology, Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany.

Claudia Arndt (C)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.
Mildred Scheel Early Career Center, Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany.

Alexandra Kegler (A)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

Manja Kubeil (M)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

Christoph E Hagemeyer (CE)

Australian Centre for Blood Diseases, Central Clinical School, Monash University, Melbourne, Australia.

Holger Stephan (H)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.

Marc Schmitz (M)

Institute of Immunology, Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany.
National Center for Tumor Diseases (NCT), Partner Site Dresden, Dresden, Germany.
German Cancer Consortium (DKTK), partner site Dresden, Dresden, Germany.
German Cancer Research Center (DKFZ), Heidelberg, Germany.

Anja Feldmann (A)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany. a.feldmann@hzdr.de.
National Center for Tumor Diseases (NCT), Partner Site Dresden, Dresden, Germany. a.feldmann@hzdr.de.
German Cancer Consortium (DKTK), partner site Dresden, Dresden, Germany. a.feldmann@hzdr.de.
German Cancer Research Center (DKFZ), Heidelberg, Germany. a.feldmann@hzdr.de.

Michael Bachmann (M)

Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany. m.bachmann@hzdr.de.
National Center for Tumor Diseases (NCT), Partner Site Dresden, Dresden, Germany. m.bachmann@hzdr.de.
German Cancer Consortium (DKTK), partner site Dresden, Dresden, Germany. m.bachmann@hzdr.de.
German Cancer Research Center (DKFZ), Heidelberg, Germany. m.bachmann@hzdr.de.

Classifications MeSH