Novel diagnostic approach for amoebic liver abscess using cell free (cf) DNA: a prospective study.
Amoebic liver abscess
cell free DNA
real-time PCR
serum
urine
Journal
Infectious diseases (London, England)
ISSN: 2374-4243
Titre abrégé: Infect Dis (Lond)
Pays: England
ID NLM: 101650235
Informations de publication
Date de publication:
Apr 2024
Apr 2024
Historique:
medline:
18
3
2024
pubmed:
19
12
2023
entrez:
19
12
2023
Statut:
ppublish
Résumé
Amoebic liver abscess (ALA) is commonly seen in tropical countries and diagnosis of ALA relies mainly on non-specific serological and imaging techniques as well as PCR from pus. This study evaluated the potential of using cell free DNA (cfDNA) from serum and urine for diagnosing ALA. We prospectively evaluated quantitative PCR (qPCR) for detection of cf DNA in serum and urine sample in all liver abscess patients. The samples were collected from patients reporting to emergency ward of Postgraduate Institute of Medical Education and Research, Chandigarh, India with symptoms suggestive of liver abscess. Real time PCR was done to detect cf DNA in serum and urine by targeting 99-bp unit of A total 113 samples (serum and urine) and 100 pus samples were analysed. A total of 62 ALA patients were confirmed; with maximum 57 patients detected by qPCR for cfDNA in the serum, 55 patients by PCR on pus aspirate and 50 ALA patients by qPCR for cfDNA in urine sample. Therefore, the sensitivity of qPCR for detection of cf DNA in serum was 91.94% and for urine was 80.65%. A total of 11.2% of ALA patients were diagnosed only through detection of
Sections du résumé
BACKGROUND
UNASSIGNED
Amoebic liver abscess (ALA) is commonly seen in tropical countries and diagnosis of ALA relies mainly on non-specific serological and imaging techniques as well as PCR from pus.
OBJECTIVE
UNASSIGNED
This study evaluated the potential of using cell free DNA (cfDNA) from serum and urine for diagnosing ALA.
METHODS
UNASSIGNED
We prospectively evaluated quantitative PCR (qPCR) for detection of cf DNA in serum and urine sample in all liver abscess patients. The samples were collected from patients reporting to emergency ward of Postgraduate Institute of Medical Education and Research, Chandigarh, India with symptoms suggestive of liver abscess. Real time PCR was done to detect cf DNA in serum and urine by targeting 99-bp unit of
RESULTS
UNASSIGNED
A total 113 samples (serum and urine) and 100 pus samples were analysed. A total of 62 ALA patients were confirmed; with maximum 57 patients detected by qPCR for cfDNA in the serum, 55 patients by PCR on pus aspirate and 50 ALA patients by qPCR for cfDNA in urine sample. Therefore, the sensitivity of qPCR for detection of cf DNA in serum was 91.94% and for urine was 80.65%.
CONCLUSION
UNASSIGNED
A total of 11.2% of ALA patients were diagnosed only through detection of
Identifiants
pubmed: 38112684
doi: 10.1080/23744235.2023.2294119
doi:
Substances chimiques
DNA, Protozoan
0
Cell-Free Nucleic Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM