White blood cell subtypes and neutrophil extracellular traps content as biomarkers for stroke etiology in acute ischemic stroke clots retrieved by mechanical thrombectomy.

Etiology Ischemic stroke Lymphocytes Macrophages Neutrophil extracellular traps Neutrophils White blood cells

Journal

Thrombosis research

ISSN: 1879-2472

Titre abrégé: Thromb Res

Pays: United States

ID NLM: 0326377

Informations de publication

Date de publication:
15 Dec 2023

Historique:

received: 01 09 2023

revised: 17 11 2023

accepted: 12 12 2023

medline: 19 12 2023

pubmed: 19 12 2023

entrez: 19 12 2023

Statut: aheadofprint

Résumé

Lymphocytes, macrophages, neutrophils, and neutrophil extracellular traps (NETs) associate with stroke risk factors and form a thrombus through different mechanisms. We investigated the total WBCs, WBC subtypes and NETs composition in acute ischemic stroke (AIS) clots to identify possible etiological differences that could help us further understand the process of thrombosis that leads to AIS. AIS clots from 100 cases each of atherothrombotic (AT), cardioembolic (CE) and cryptogenic stroke etiology were collected per-pass as part of the CÚRAM RESTORE registry of AIS clots. Martius Scarlet Blue stain was used to identify the main histological components of the clots. Immunohistochemical staining was used to identify neutrophils, lymphocytes, macrophages, and NETs patterns. The cellular and histological components were quantified using Orbit Image Analysis software. AT clots were larger, with more red blood cells and fewer WBCs than CE clots. AT clots had more lymphocytes and cryptogenic clots had fewer macrophages than other etiologies. Most significantly, CE clots showed higher expression of neutrophils and extracellular web-like NETs compared to AT and cryptogenic clots. There was also a significantly higher distribution of web-like NETs around the periphery of the CE clots while a mixed distribution was observed in AT clots. The difference in neutrophil and NETs expression in clots from different etiologies may provide insight into the mechanism of clot formation.

Sections du résumé

BACKGROUND BACKGROUND

METHODS METHODS

AIS clots from 100 cases each of atherothrombotic (AT), cardioembolic (CE) and cryptogenic stroke etiology were collected per-pass as part of the CÚRAM RESTORE registry of AIS clots. Martius Scarlet Blue stain was used to identify the main histological components of the clots. Immunohistochemical staining was used to identify neutrophils, lymphocytes, macrophages, and NETs patterns. The cellular and histological components were quantified using Orbit Image Analysis software.

RESULTS RESULTS

AT clots were larger, with more red blood cells and fewer WBCs than CE clots. AT clots had more lymphocytes and cryptogenic clots had fewer macrophages than other etiologies. Most significantly, CE clots showed higher expression of neutrophils and extracellular web-like NETs compared to AT and cryptogenic clots. There was also a significantly higher distribution of web-like NETs around the periphery of the CE clots while a mixed distribution was observed in AT clots.

CONCLUSION CONCLUSIONS

The difference in neutrophil and NETs expression in clots from different etiologies may provide insight into the mechanism of clot formation.

Identifiants

DOI: 10.1016/j.thromres.2023.12.005 PMID: 38113606

pubmed: 38113606

pii: S0049-3848(23)00352-3

doi: 10.1016/j.thromres.2023.12.005

pii:

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

1-8

Informations de copyright

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.