Protocol for using UV stimuli to evoke prey capture strikes in head-fixed zebrafish larvae.

Behavior Model Organisms Neuroscience

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
15 Mar 2024
Historique:
received: 21 09 2023
revised: 21 10 2023
accepted: 01 12 2023
pubmed: 20 12 2023
medline: 20 12 2023
entrez: 20 12 2023
Statut: ppublish

Résumé

Hunting in larval zebrafish begins with eye convergence and orienting turns, proceeds to approach swims, and ends with the strike, where larvae consume the prey. Here, we describe a protocol to present UV stimuli to zebrafish, which greatly increases the occurrence of hunting initiation and strikes. We also describe how we record and analyze strike behavior in head-fixed larvae. Our goals are to increase the robustness of prey capture and to allow other labs to implement the strike behavioral assay. For complete details on the use and execution of this protocol, please refer to Khan et al. (2023).

Identifiants

DOI: 10.1016/j.xpro.2023.102780 PMID: 38117657 PMC: PMC10772384
pubmed: 38117657
pii: S2666-1667(23)00747-5
doi: 10.1016/j.xpro.2023.102780
pmc: PMC10772384
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102780

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Biswadeep Khan (B)

Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong S.A.R., China. Electronic address: biswadeep.khan@connect.ust.hk.

Ivan P Lazarte (IP)

Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong S.A.R., China; Department of Physiology, Anatomy and Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford OX1 3PT, UK.

On-Mongkol Jaesiri (OM)

Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong S.A.R., China.

Peixiong Zhao (P)

Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong S.A.R., China.

Julie L Semmelhack (JL)

Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong S.A.R., China; Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong S.A.R., China. Electronic address: jsemmelhack@ust.hk.

Classifications MeSH