Flavonoids are involved in salt tolerance through ROS scavenging in the halophyte Atriplex canescens.
Arabidopsis thaliana
Atriplex canescens
Chalcone isomerase
Flavonoids
Salt and drought tolerance
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
21 Dec 2023
21 Dec 2023
Historique:
received:
05
07
2023
accepted:
26
09
2023
medline:
21
12
2023
pubmed:
21
12
2023
entrez:
21
12
2023
Statut:
epublish
Résumé
The content of flavonoids could increase in A. canescens under saline conditions. Overexpression of AcCHI in transgenic A. thaliana promotes flavonoid biosynthesis, thereby functioning in the tolerance of transgenic plants to salt and osmotic stress by maintaining ROS homeostasis. Atriplex canescens is a halophytic forage shrub with excellent adaptation to saline environment. Our previous study showed that a large number of genes related to the biosynthesis of flavonoids in A. canescens were significantly up-regulated by NaCl treatments. However, it remains unclear whether flavonoids are involved in A. canescens response to salinity. In this study, we found that the accumulation of flavonoids significantly increased in either the leaves or roots of A. canescens seedling under 100 and 300 mM NaCl treatments. Correspondingly, AcCHS, AcCHI and AcF3H, which encode three key enzymes (chalcone synthases (CHS), chalcone isomerase (CHI), and flavanone 3-hydroxylase (F3H), respectively) of flavonoids biosynthesis, were significantly induced in the roots or leaves of A. canescens by 100 or 300 mM NaCl. Then, we generated the transgenic Arabidopsis thaliana overexpressing AcCHI and found that transgenic plants accumulated more flavonoids through enhancing the pathway of flavonoids biosynthesis. Furthermore, overexpression of AcCHI conferred salt and osmotic stress tolerance in transgenic A. thaliana. Contrasted with wild-type A. thaliana, transgenic lines grew better with greater biomass, less H
Identifiants
pubmed: 38127154
doi: 10.1007/s00299-023-03087-6
pii: 10.1007/s00299-023-03087-6
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5Subventions
Organisme : the National Natural Science Foundation of China
ID : 31971761
Organisme : the National Natural Science Foundation of China
ID : 32371756
Organisme : the Key Science & Technology Project of Gansu Province
ID : 22ZD6NA007
Organisme : the Science & Technology Project from China Huaneng Group Co. LTD.
ID : HNKJ21-H76
Organisme : the Fundamental Research Funds for the Central Universities
ID : lzujbky-2022-ct03
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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