Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6).
BoHV6
ELISA
protein expression
recombinant glycoprotein B (rgB)
rgH
Journal
Journal of veterinary research
ISSN: 2450-7393
Titre abrégé: J Vet Res
Pays: Poland
ID NLM: 101696630
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
08
10
2023
accepted:
05
12
2023
medline:
22
12
2023
pubmed:
22
12
2023
entrez:
22
12
2023
Statut:
epublish
Résumé
Bovine herpesvirus 6 (BoHV6) belongs to the Viral DNA from a BoHV6-positive cow was amplified by qPCR and the resulting fragments of the The proteins expressed had the expected molecular weight. A total of 143 sera were examined, and 141 of them were positive, according to the chosen cut-off values of 9% and 10% for the sample-to-positive ratios of the rgB and rgH antigens, respectively. The rgB and rgH recombinant antigens of BoHV6 were successfully expressed in
Identifiants
pubmed: 38130460
doi: 10.2478/jvetres-2023-0069
pii: jvetres-2023-0069
pmc: PMC10730543
doi:
Types de publication
Journal Article
Langues
eng
Pagination
509-515Informations de copyright
© 2023 Piotr Kubiś et al., published by Sciendo.
Déclaration de conflit d'intérêts
Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.