Disruption of a Plasmodium falciparum patatin-like phospholipase delays male gametocyte exflagellation.
egress
exflagellation
gametocyte
malaria
phospholipase
Journal
Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028
Informations de publication
Date de publication:
22 Dec 2023
22 Dec 2023
Historique:
revised:
01
12
2023
received:
28
04
2023
accepted:
03
12
2023
medline:
22
12
2023
pubmed:
22
12
2023
entrez:
22
12
2023
Statut:
aheadofprint
Résumé
An essential process in transmission of the malaria parasite to the Anopheles vector is the conversion of mature gametocytes into gametes within the mosquito gut, where they egress from the red blood cell (RBC). During egress, male gametocytes undergo exflagellation, leading to the formation of eight haploid motile microgametes, while female gametes retain their spherical shape. Gametocyte egress depends on sequential disruption of the parasitophorous vacuole membrane and the host cell membrane. In other life cycle stages of the malaria parasite, phospholipases have been implicated in membrane disruption processes during egress, however their importance for gametocyte egress is relatively unknown. Here, we performed comprehensive functional analyses of six putative phospholipases for their role during development and egress of Plasmodium falciparum gametocytes. We localize two of them, the prodrug activation and resistance esterase (PF3D7_0709700) and the lysophospholipase 1 (PF3D7_1476700), to the parasite plasma membrane. Subsequently, we show that disruption of most of the studied phospholipase genes does neither affect gametocyte development nor egress. The exception is the putative patatin-like phospholipase 3 (PF3D7_0924000), whose gene deletion leads to a delay in male gametocyte exflagellation, indicating an important, albeit not essential, role of this enzyme in male gametogenesis.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Alexander von Humboldt-Stiftung
Organisme : Australian Research Council
Organisme : DAAD/Universities Australia
Organisme : Deutsche Forschungsgemeinschaft
ID : 414222880
Organisme : Deutsche Forschungsgemeinschaft
ID : 446556156
Organisme : DFG within the SPP2225
Informations de copyright
© 2023 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.
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