Feasibility Study Utilizing NanoString's Digital Spatial Profiling (DSP) Technology for Characterizing the Immune Microenvironment in Barrett's Esophagus Formalin-Fixed Paraffin-Embedded Tissues.
Barrett’s esophagus
esophageal adenocarcinoma
immune profiling
spatial proteomics
spatial transcriptomics
tumor microenvironment
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
18 Dec 2023
18 Dec 2023
Historique:
received:
17
11
2023
revised:
14
12
2023
accepted:
14
12
2023
medline:
23
12
2023
pubmed:
23
12
2023
entrez:
23
12
2023
Statut:
epublish
Résumé
Characterization of the Barrett's esophagus (BE) microenvironment in patients with a known progression status, to determine how it may influence BE progression to esophageal adenocarcinoma (EAC), has been understudied, hindering both the biological understanding of the progression and the development of novel diagnostics and therapies. This study's aim was to determine if a highly multiplex interrogation of the microenvironment can be performed on endoscopic formalin-fixed, paraffin-embedded (FFPE) samples, utilizing the NanoString GeoMx digital spatial profiling (GeoMx DSP) platform and if it can begin to identify the types of immune cells and pathways that may mediate the progression of BE. We performed a spatial proteomic analysis of 49 proteins expressed in the microenvironment and epithelial cells of FFPE endoscopic biopsies from patients with non-dysplastic BE (NDBE) who later progressed to high-grade dysplasia or EAC
Identifiants
pubmed: 38136440
pii: cancers15245895
doi: 10.3390/cancers15245895
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : American Cancer Society
ID : UCSF Pilot Award
Organisme : National Institute of Health
ID : NIDDK K08DK109209
Organisme : National Institute of Health
ID : NCI R37CA269649