Stimulation of Hemolysis and Eryptosis by β-Caryophyllene Oxide.
calcium
eryptosis
hemolysis
oxidative stress
β-caryophyllene oxide
Journal
Life (Basel, Switzerland)
ISSN: 2075-1729
Titre abrégé: Life (Basel)
Pays: Switzerland
ID NLM: 101580444
Informations de publication
Date de publication:
04 Dec 2023
04 Dec 2023
Historique:
received:
18
10
2023
revised:
27
11
2023
accepted:
28
11
2023
medline:
23
12
2023
pubmed:
23
12
2023
entrez:
23
12
2023
Statut:
epublish
Résumé
Eryptosis stimulated by anticancer drugs can lead to anemia in patients. β-caryophyllene oxide (CPO) is an anticancer sesquiterpene present in various plants; however, its effect on the structure and function of human red blood cells (RBCs) remains unexplored. The aim of this study was to investigate the hemolytic and eryptotic activities and underlying molecular mechanisms of CPO in human RBCs. Cells were treated with 10-100 μM of CPO for 24 h at 37 °C, and hemolysis, LDH, AST, and AChE activities were photometrically assayed. Flow cytometry was employed to determine changes in cell volume from FSC, phosphatidylserine (PS) externalization by annexin-V-FITC, intracellular calcium by Fluo4/AM, and oxidative stress by 2',7'-dichlorodihydrofluorescein diacetate (H CPO induced concentration-responsive hemolysis with LDH and AST leakage, in addition to PS exposure, cell shrinkage, Ca CPO stimulates hemolysis and eryptosis through energy depletion, Ca
Sections du résumé
BACKGROUND
BACKGROUND
Eryptosis stimulated by anticancer drugs can lead to anemia in patients. β-caryophyllene oxide (CPO) is an anticancer sesquiterpene present in various plants; however, its effect on the structure and function of human red blood cells (RBCs) remains unexplored. The aim of this study was to investigate the hemolytic and eryptotic activities and underlying molecular mechanisms of CPO in human RBCs.
METHODS
METHODS
Cells were treated with 10-100 μM of CPO for 24 h at 37 °C, and hemolysis, LDH, AST, and AChE activities were photometrically assayed. Flow cytometry was employed to determine changes in cell volume from FSC, phosphatidylserine (PS) externalization by annexin-V-FITC, intracellular calcium by Fluo4/AM, and oxidative stress by 2',7'-dichlorodihydrofluorescein diacetate (H
RESULTS
RESULTS
CPO induced concentration-responsive hemolysis with LDH and AST leakage, in addition to PS exposure, cell shrinkage, Ca
CONCLUSIONS
CONCLUSIONS
CPO stimulates hemolysis and eryptosis through energy depletion, Ca
Identifiants
pubmed: 38137900
pii: life13122299
doi: 10.3390/life13122299
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Deputyship for Research and Innovation, Ministry of Education, Saudi Arabia
ID : IFKSUDR-H236