Endoplasmic Reticulum Calcium Mediates
BMP
Drosophila wing development
Orai
SERCA
Stim
bestrophin
bone morphogenic protein
calcium
endoplasmic reticulum
transients
wing venation
Journal
Bioelectricity
ISSN: 2576-3113
Titre abrégé: Bioelectricity
Pays: United States
ID NLM: 101747121
Informations de publication
Date de publication:
01 Dec 2023
01 Dec 2023
Historique:
pmc-release:
01
12
2024
medline:
25
12
2023
pubmed:
25
12
2023
entrez:
25
12
2023
Statut:
ppublish
Résumé
The temporal dynamics of morphogen presentation impacts transcriptional responses and tissue patterning. However, the mechanisms controlling morphogen release are far from clear. We found that inwardly rectifying potassium (Irk) channels regulate endogenous transient increases in intracellular calcium and bone morphogenetic protein (BMP/Dpp) release for To test this hypothesis, we reduced expression of four proteins that control ER calcium, Stromal interaction molecule 1 (Stim), Calcium release-activated calcium channel protein 1 (Orai), SarcoEndoplasmic Reticulum Calcium ATPase (SERCA), small conductance calcium-activated potassium channel (SK), and Bestrophin 2 (Best2) using RNAi and documented wing phenotypes. We use live imaging to study calcium and Dpp release within pupal wings and larval wing discs. Additionally, we employed immunohistochemistry to characterize Small Mothers Against Decapentaplegic (SMAD) phosphorylation downstream of the BMP/Dpp pathway following RNAi knockdown. We found that reduced Stim and SERCA function decreases amplitude and frequency of endogenous calcium transients in the wing disc and reduced BMP/Dpp release. Our results suggest control of ER calcium homeostasis is required for BMP/Dpp release, and
Sections du résumé
Background
UNASSIGNED
The temporal dynamics of morphogen presentation impacts transcriptional responses and tissue patterning. However, the mechanisms controlling morphogen release are far from clear. We found that inwardly rectifying potassium (Irk) channels regulate endogenous transient increases in intracellular calcium and bone morphogenetic protein (BMP/Dpp) release for
Materials and Methods
UNASSIGNED
To test this hypothesis, we reduced expression of four proteins that control ER calcium, Stromal interaction molecule 1 (Stim), Calcium release-activated calcium channel protein 1 (Orai), SarcoEndoplasmic Reticulum Calcium ATPase (SERCA), small conductance calcium-activated potassium channel (SK), and Bestrophin 2 (Best2) using RNAi and documented wing phenotypes. We use live imaging to study calcium and Dpp release within pupal wings and larval wing discs. Additionally, we employed immunohistochemistry to characterize Small Mothers Against Decapentaplegic (SMAD) phosphorylation downstream of the BMP/Dpp pathway following RNAi knockdown.
Results
UNASSIGNED
We found that reduced Stim and SERCA function decreases amplitude and frequency of endogenous calcium transients in the wing disc and reduced BMP/Dpp release.
Conclusion
UNASSIGNED
Our results suggest control of ER calcium homeostasis is required for BMP/Dpp release, and
Identifiants
pubmed: 38143873
doi: 10.1089/bioe.2022.0036
pii: 10.1089/bioe.2022.0036
pmc: PMC10733776
doi:
Types de publication
Journal Article
Langues
eng
Pagination
290-306Informations de copyright
Copyright 2023, Mary Ann Liebert, Inc., publishers.
Déclaration de conflit d'intérêts
No competing financial interests exist.