C3d Immunohistochemical Staining on Paraffin-Embedded Tissue for Diagnosis of Pemphigus.

Journal

Archives of pathology & laboratory medicine
ISSN: 1543-2165
Titre abrégé: Arch Pathol Lab Med
Pays: United States
ID NLM: 7607091

Informations de publication

Date de publication:
27 Dec 2023
Historique:
accepted: 09 10 2023
medline: 27 12 2023
pubmed: 27 12 2023
entrez: 27 12 2023
Statut: aheadofprint

Résumé

Pemphigus is an autoimmune blister disease that causes blisters on the skin and mucosal surfaces. Direct immunofluorescence (DIF) testing is critical for the clinical diagnosis of pemphigus. However, it is limited to fresh tissue specimens and fluorescence microscopy. To assess the value of C3d immunohistochemistry (IHC) on paraffin-embedded skin tissue for the diagnosis of pemphigus by comparing C3d-IHC results to DIF and enzyme-linked immunosorbent assay testing in pemphigus and other blister-related skin diseases. C3d-IHC assays were retrospectively performed on paraffin-embedded skin tissue sections from 115 patients (63 with pemphigus and 52 controls). Both the case group and the control group underwent the same protocol, and cases with C3d position in the peripheral spinous layer were considered as positive samples. C3d-IHC and DIF testing had similar performance for pemphigus diagnosis, with a sensitivity of 71.0% (95% CI, 51.8%-85.1%) and 77.4% (95% CI, 58.5%-89.7%), specificity of 96.4% (95% CI, 79.8%-99.8%) and 100% (95% CI, 85.0%-100%), positive predictive value of 95.7% (95% CI, 76.0%-99.8%) and 100% (95% CI, 82.8%-100%), and a negative predictive value of 75.0% (95% CI, 57.5%-87.3%) and 80.0% (95% CI, 62.5%-90.9%), respectively. Our study indicated that C3d-IHC results for paraffin-fixed tissues were not significantly different from DIF results for the diagnosis of pemphigus. The C3d-IHC assay has the potential for routine diagnosis of pemphigus, especially in the absence of fresh-frozen tissue.

Identifiants

DOI: 10.5858/arpa.2023-0274-OA PMID: 38149406
pubmed: 38149406
pii: 497890
doi: 10.5858/arpa.2023-0274-OA
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2023 College of American Pathologists.

Déclaration de conflit d'intérêts

The authors have no relevant financial interest in the products or companies described in this article.

Auteurs

Nanhui Wu (N)

From the Departments of Dermatopathology (N Wu, Cai, F Wu, Liang, S Liu, Y Liu) and Central Laboratory (Zhang), Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Cai is currently located at the Department of Dermatopathology, Shanghai East Hospital, Shanghai, China. Authors N Wu, Cai, and F Wu contributed equally to this manuscript.

Yijie Cai (Y)

From the Departments of Dermatopathology (N Wu, Cai, F Wu, Liang, S Liu, Y Liu) and Central Laboratory (Zhang), Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Cai is currently located at the Department of Dermatopathology, Shanghai East Hospital, Shanghai, China. Authors N Wu, Cai, and F Wu contributed equally to this manuscript.

Fei Wu (F)

From the Departments of Dermatopathology (N Wu, Cai, F Wu, Liang, S Liu, Y Liu) and Central Laboratory (Zhang), Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Cai is currently located at the Department of Dermatopathology, Shanghai East Hospital, Shanghai, China. Authors N Wu, Cai, and F Wu contributed equally to this manuscript.

Yulin Liang (Y)

From the Departments of Dermatopathology (N Wu, Cai, F Wu, Liang, S Liu, Y Liu) and Central Laboratory (Zhang), Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Cai is currently located at the Department of Dermatopathology, Shanghai East Hospital, Shanghai, China. Authors N Wu, Cai, and F Wu contributed equally to this manuscript.

Shuyi Liu (S)

From the Departments of Dermatopathology (N Wu, Cai, F Wu, Liang, S Liu, Y Liu) and Central Laboratory (Zhang), Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Cai is currently located at the Department of Dermatopathology, Shanghai East Hospital, Shanghai, China. Authors N Wu, Cai, and F Wu contributed equally to this manuscript.

Pengfei Zhang (P)

From the Departments of Dermatopathology (N Wu, Cai, F Wu, Liang, S Liu, Y Liu) and Central Laboratory (Zhang), Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Cai is currently located at the Department of Dermatopathology, Shanghai East Hospital, Shanghai, China. Authors N Wu, Cai, and F Wu contributed equally to this manuscript.

Yeqiang Liu (Y)

From the Departments of Dermatopathology (N Wu, Cai, F Wu, Liang, S Liu, Y Liu) and Central Laboratory (Zhang), Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Cai is currently located at the Department of Dermatopathology, Shanghai East Hospital, Shanghai, China. Authors N Wu, Cai, and F Wu contributed equally to this manuscript.

Classifications MeSH