The regenerative potential of Tideglusib and CHIR99021 small molecules as potent odontogenic differentiation enhancers of human dental pulp stem cells.

AXIN2 Dental pulp capping Dentin GSK-3 inhibitor Regeneration Wnt/β-catenin signalling pathway

Journal

Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115

Informations de publication

Date de publication:
28 Dec 2023
Historique:
received: 07 10 2023
accepted: 18 12 2023
medline: 28 12 2023
pubmed: 28 12 2023
entrez: 28 12 2023
Statut: epublish

Résumé

To assess the effect of Tideglusib and CHIR99021 small molecules on the odontogenic differentiation potential of human dental pulp stem cells (hDPSCs) via Wnt/β-catenin pathway activation. hDPSCs were isolated from impacted third molars indicated for extraction and were characterized by flow cytometry. hDPSCs were then induced to differentiate into odontogenic lineage in the presence of Tideglusib and CHIR99021. Odontogenic differentiation was evaluated using Alizarin Red stain and RT-PCR for expression of odontogenic specific differentiation markers: DSPP, DMP1, ALP, OPN, and RUNX2 in relation to undifferentiated cells. RT-PCR was also conducted to assess the expression of Wnt/β-catenin pathway activation marker (AXIN2). One-way ANOVA Kruskal-Wallis test was used for statistical analysis. Wnt/β-catenin pathway was successfully activated by Tideglusib and CHIR99021 in hDPSCs where AXIN2 was significantly upregulated. Successful odontogenic differentiation was confirmed by Alizarin Red staining of calcified nodules. RT-PCR for odontogenic differentiation markers DSPP, DMP1, and RUNX expression by hDPSCs induced by CHIR99021 was higher than that expressed by hDPSCs induced by Tideglusib, whereas expression of OPN and ALP was higher in Tideglusib-induced cells than in CHIR99021-induced cells. Both small molecules successfully induced odontogenic differentiation of hDPSCs through Wnt/β-catenin pathway activation. These findings suggest that Tideglusib and CHIR99021 can be applied clinically in pulp regeneration to improve strategies for vital pulp regeneration and to promote dentine repair.

Identifiants

DOI: 10.1007/s00784-023-05452-x PMID: 38153556
pubmed: 38153556
doi: 10.1007/s00784-023-05452-x
pii: 10.1007/s00784-023-05452-x
doi:

Types de publication

Journal Article

Langues

eng

Pagination

48

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

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Auteurs

Samer Hanna (S)

Endodontics Department, Universidad Europea De Madrid (UEM), Madrid, Spain.

Ghada Nour Eldeen (GN)

Molecular Genetics & Enzymology Department, Human Genetic & Genome Research Institute, National Research Centre, Dokki, Giza, Egypt.

Ruth Perez Alfayate (RP)

Endodontics Department, Universidad Europea De Madrid (UEM), Madrid, Spain.

Riham Aly (R)

Basic Dental Science Department, Oral Medicine & Dentistry Research Institute, National Research Centre, Dokki, Giza, Egypt. rm.el-sayed@nrc.sci.eg.
Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt. rm.el-sayed@nrc.sci.eg.

Classifications MeSH