Chronic oxytocin administration stimulates the oxytocinergic system in children with autism.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
02 Jan 2024
Historique:
received: 05 06 2023
accepted: 08 12 2023
medline: 4 1 2024
pubmed: 4 1 2024
entrez: 3 1 2024
Statut: epublish

Résumé

Clinical efficacy of intranasal administration of oxytocin is increasingly explored in autism spectrum disorder, but to date, the biological effects of chronic administration regimes on endogenous oxytocinergic function are largely unknown. Here exploratory biological assessments from a completed randomized, placebo-controlled trial showed that children with autism (n = 79, 16 females) receiving intranasal oxytocin for four weeks (12 IU, twice daily) displayed significantly higher salivary oxytocin levels 24 hours after the last oxytocin nasal spray administration, but no longer at a four-week follow up session. Regarding salivary oxytocin receptor gene (OXTR) epigenetics (DNA-methylation), oxytocin-induced reductions in OXTR DNA-methylation were observed, suggesting a facilitation of oxytocin receptor expression in the oxytocin compared to the placebo group. Notably, heightened oxytocin levels post-treatment were significantly associated with reduced OXTR DNA-methylation and improved feelings of secure attachment. These findings indicate that four weeks of chronic oxytocin administration stimulated the endogenous oxytocinergic system in children with autism.

Identifiants

pubmed: 38167302
doi: 10.1038/s41467-023-44334-4
pii: 10.1038/s41467-023-44334-4
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

58

Informations de copyright

© 2024. The Author(s).

Références

American Psychiatric Association. Diagnostic and statistical manual of mental disorders (5th ed). Washington, DC (2013).
Ooi, Y. P., Weng, S. J., Kossowsky, J., Gerger, H. & Sung, M. Oxytocin and Autism Spectrum Disorders: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Pharmacopsychiatry 50, 5–13 (2017).
pubmed: 27574858
Bethlehem, R. A. I., Baron-Cohen, S., van Honk, J., Auyeung, B. & Bos, P. A. The oxytocin paradox. Front Behav. Neurosci. 8, 5 (2014).
doi: 10.3389/fnbeh.2014.00048
Ma, Y., Shamay-Tsoory, S., Han, S. & Zink, C. F. Oxytocin and Social Adaptation: Insights from Neuroimaging Studies of Healthy and Clinical Populations. Trends Cogn. Sci. 20, 133–145 (2016).
pubmed: 26616296 doi: 10.1016/j.tics.2015.10.009
Quintana, D. S. et al. Low-dose oxytocin delivered intranasally with Breath Powered device affects social-cognitive behavior: a randomized four-way crossover trial with nasal cavity dimension assessment. Transl. Psychiatry 5, e602–e602 (2015).
pubmed: 26171983 pmcid: 5068727 doi: 10.1038/tp.2015.93
Stoop, R. Neuromodulation by oxytocin and vasopressin in the central nervous system as a basis for their rapid behavioral effects. Curr. Opin. Neurobiol. 29, 187–193 (2014).
pubmed: 25463629 doi: 10.1016/j.conb.2014.09.012
Shamay-Tsoory, S. G. & Abu-Akel, A. The Social Salience Hypothesis of Oxytocin. Biol. Psychiatry 79, 194–202 (2016).
pubmed: 26321019 doi: 10.1016/j.biopsych.2015.07.020
Anagnostou, E. et al. Intranasal oxytocin versus placebo in the treatment of adults with autism spectrum disorders: A randomized controlled trial. Mol. Autism 3, 1–9 (2012).
doi: 10.1186/2040-2392-3-16
Bernaerts, S., Boets, B., Bosmans, G., Steyaert, J. & Alaerts, K. Behavioral effects of multiple-dose oxytocin treatment in autism: A randomized, placebo-controlled trial with long-term follow-up. Mol. Autism 11, 1–14 (2020).
doi: 10.1186/s13229-020-0313-1
Le, J. et al. Infrequent Intranasal Oxytocin Followed by Positive Social Interaction Improves Symptoms in Autistic Children: A Pilot Randomized Clinical Trial. Psychother Psychosom 1–13 (2022).
Sikich, L. et al. Intranasal Oxytocin in Children and Adolescents with Autism Spectrum Disorder. N. Engl. J. Med. 385, 1462–1473 (2021).
pubmed: 34644471 pmcid: 9701092 doi: 10.1056/NEJMoa2103583
Daniels, N. et al. Effects of multiple-dose intranasal oxytocin administration on social responsiveness in children with autism: a randomized, placebo-controlled trial. Mol. Autism 14, 16 (2023).
pubmed: 37081454 pmcid: 10117268 doi: 10.1186/s13229-023-00546-5
Horta, M., Kaylor, K., Feifel, D. & Ebner, N. C. Chronic oxytocin administration as a tool for investigation and treatment: A cross-disciplinary systematic review. Neurosci. Biobehav. Rev. 108, 1–23 (2020).
pubmed: 31647964 doi: 10.1016/j.neubiorev.2019.10.012
Moerkerke, M. et al. Endogenous Oxytocin Levels in Autism—A Meta-Analysis. Brain Sci. 2021 11, 1545 (2021).
Evenepoel, M. et al. Endogenous oxytocin levels in children with autism: Associations with cortisol levels and oxytocin receptor gene methylation. Transl. Psychiatry 13, 1–9 (2023). 2023 13:1.
doi: 10.1038/s41398-023-02524-0
John, S. & Jaeggi, A. V. Oxytocin levels tend to be lower in autistic children: A meta-analysis of 31 studies. Autism 25, 2152–2161 (2021).
pubmed: 34308675 doi: 10.1177/13623613211034375
Parker, K. J. et al. Intranasal oxytocin treatment for social deficits and biomarkers of response in children with autism. Proc. Natl. Acad. Sci. USA 114, 8119–8124 (2017).
pubmed: 28696286 pmcid: 5544319 doi: 10.1073/pnas.1705521114
Daughters, K. et al. Salivary Oxytocin Concentrations in Males following Intranasal Administration of Oxytocin: A Double-Blind, Cross-Over Study. (2015).
Huffmeijer, R. et al. Salivary levels of oxytocin remain elevated for more than two hours after intranasal oxytocin administration. Neuroendocrinol. Lett. 33, 22467107–330112 (2012).
Quintana, D. S. et al. Saliva oxytocin measures do not reflect peripheral plasma concentrations after intranasal oxytocin administration in men. Horm. Behav. 102, 85–92 (2018).
pubmed: 29750971 doi: 10.1016/j.yhbeh.2018.05.004
Riem, M. M. E., van IJzendoorn, M. H. & Bakermans-Kranenburg, M. J. Hippocampal volume modulates salivary oxytocin level increases after intranasal oxytocin administration. Psychoneuroendocrinology 101, 182–185 (2019).
pubmed: 30469085 doi: 10.1016/j.psyneuen.2018.11.015
Weisman, O., Zagoory-Sharon, O. & Feldman, R. Intranasal oxytocin administration is reflected in human saliva. Psychoneuroendocrinology 37, 1582–1586 (2012).
pubmed: 22436536 doi: 10.1016/j.psyneuen.2012.02.014
Procyshyn, T. L. et al. Effects of oxytocin administration on salivary sex hormone levels in autistic and neurotypical women. Mol. Autism 11, 1–11 (2020).
doi: 10.1186/s13229-020-00326-5
van IJzendoorn, M. H., Bhandari, R., van der Veen, R., Grewen, K. M. & Bakermans-Kranenburg, M. J. Elevated Salivary Levels of Oxytocin Persist More than 7 h after Intranasal Administration. Front Neurosci. 6, 174 (2012).
pubmed: 23233832 pmcid: 3516702
Jurek, B. & Neumann, I. D. The oxytocin receptor: From intracellular signaling to behavior. Physiol. Rev. 98, 1805–1908 (2018).
pubmed: 29897293 doi: 10.1152/physrev.00031.2017
Alaerts, K., Steyaert, J., Vanaudenaerde, B., Wenderoth, N. & Bernaerts, S. Changes in endogenous oxytocin levels after intranasal oxytocin treatment in adult men with autism: An exploratory study with long-term follow-up. Eur. Neuropsychopharmacol. 43, 147–152 (2021).
pubmed: 33309460 doi: 10.1016/j.euroneuro.2020.11.014
De Dreu, C. K. W. Oxytocin modulates cooperation within and competition between groups: An integrative review and research agenda. Horm. Behav. 61, 419–428 (2012).
pubmed: 22227278 doi: 10.1016/j.yhbeh.2011.12.009
Kusui, C. et al. DNA methylation of the human oxytocin receptor gene promoter regulates tissue-specific gene suppression. Biochem Biophys. Res Commun. 289, 681–686 (2001).
pubmed: 11726201 doi: 10.1006/bbrc.2001.6024
Moerkerke, M. et al. Oxytocin receptor gene (OXTR) DNA methylation is associated with autism and related social traits – A systematic review. Res Autism Spectr. Disord. 85, 101785 (2021).
doi: 10.1016/j.rasd.2021.101785
Gregory, S. G. et al. Genomic and epigenetic evidence for oxytocin receptor deficiency in autism. BMC Med 7, 1–13 (2009).
doi: 10.1186/1741-7015-7-62
Gulliver, D. et al. Targeting the Oxytocin System: New Pharmacotherapeutic Approaches. Trends Pharm. Sci. 40, 22–37 (2019).
pubmed: 30509888 doi: 10.1016/j.tips.2018.11.001
Neumann, I., Douglas, A. J., Pittman, Q. J., Russell, J. A. & Landgraf, R. Oxytocin Released within the Supraoptic Nucleus of the Rat Brain by Positive Feedback Action is Involved in Parturition-Related Events. J. Neuroendocrinol. 8, 227–233 (1996).
pubmed: 8730656 doi: 10.1046/j.1365-2826.1996.04557.x
Ludwig, M. et al. Intracellular calcium stores regulate activity-dependent neuropeptide release from dendrites. Nature 418, 85–89 (2002).
pubmed: 12097911 doi: 10.1038/nature00822
Huang, H. et al. Chronic and Acute Intranasal Oxytocin Produce Divergent Social Effects in Mice. Neuropsychopharmacology 39, 1102–1114 (2013).
pubmed: 24190025 pmcid: 3957104 doi: 10.1038/npp.2013.310
Peters, S., Slattery, D. A., Uschold-Schmidt, N., Reber, S. O. & Neumann, I. D. Dose-dependent effects of chronic central infusion of oxytocin on anxiety, oxytocin receptor binding and stress-related parameters in mice. Psychoneuroendocrinology 42, 225–236 (2014).
pubmed: 24636519 doi: 10.1016/j.psyneuen.2014.01.021
Zimmermann-Peruzatto, J. M. et al. The Impact of Oxytocin Gene Knockout on Sexual Behavior and Gene Expression Related to Neuroendocrine Systems in the Brain of Female Mice. Cell Mol. Neurobiol. 37, 803–815 (2017).
pubmed: 27558735 doi: 10.1007/s10571-016-0419-3
Ford, C. L. & Young, L. J. Refining oxytocin therapy for autism: context is key. Nat. Rev. Neurol. 18, 67–68 (2021). 2021 18:2.
doi: 10.1038/s41582-021-00602-9
Itskovich, E., Bowling, D. L., Garner, J. P. & Parker, K. J. Oxytocin and the social facilitation of placebo effects. Molecular Psychiatry 2022 1–10 (2022).
Valstad, M. et al. The correlation between central and peripheral oxytocin concentrations: A systematic review and meta-analysis. Neurosci. Biobehav. Rev. 78, 117–124 (2017).
pubmed: 28442403 doi: 10.1016/j.neubiorev.2017.04.017
Marazziti, D. et al. A relationship between oxytocin and anxiety of romantic attachment. Clin. Pract. Epidemiol. Ment. Health 2, 1–6 (2006).
doi: 10.1186/1745-0179-2-1
Ferreira, A. C., Osório, F. & de, L. Peripheral oxytocin concentrations in psychiatric disorders – A systematic review and methanalysis: Further evidence. Prog. Neuropsychopharmacol. Biol. Psychiatry 117, 110561 (2022).
pubmed: 35461971 doi: 10.1016/j.pnpbp.2022.110561
Carter, C. S. Oxytocin pathways and the evolution of human behavior. Annu Rev. Psychol. 65, 17–39 (2014).
pubmed: 24050183 doi: 10.1146/annurev-psych-010213-115110
Porges, S. W. The Polyvagal Theory: Neurophysiological Foundations of Emotions, Attachment, Communication, Self-Regulation (Norton Series on Interpersonal Neurobiology). (WW Norton & Company., 2011).
Martin, J. et al. Oxytocin levels in saliva correlate better than plasma levels with concentrations in the cerebrospinal fluid of patients in neurocritical care. J. Neuroendocrinol. 30, e12596 (2018).
doi: 10.1111/jne.12596
Moerkerke, M. et al. Can repeated intranasal oxytocin administration affect reduced neural sensitivity towards expressive faces in autism? A randomized controlled trial. Journal of Child Psychology and Psychiatry (2023).
Alaerts, K. et al. At the Head and Heart of Oxytocin’s Stress-Regulatory Neural and Cardiac Effects: A Chronic Administration RCT in Children with Autism. Psychother Psychosom 1–14 (2023).
Lord, C. et al. ADOS-Autisme diagnostisch observatieschema Handleiding. (2012).
Constantino, J. & Gruber, C. Social responsiveness scale 2nd. ed: SRS-2. Manual. Western Psychological Services. (2012).
Wechsler, D. WISC-V-NL. Wechsler Intelligence Scale for Children, Fifth Edition, Dutch version. Preprint at (2018).
Yatawara, C. J., Einfeld, S. L., Hickie, I. B., Davenport, T. A. & Guastella, A. J. The effect of oxytocin nasal spray on social interaction deficits observed in young children with autism: A randomized clinical crossover trial. Mol. Psychiatry 21, 1225–1231 (2016).
pubmed: 26503762 doi: 10.1038/mp.2015.162
Dadds, M. R. et al. Nasal oxytocin for social deficits in childhood autism: A randomized controlled trial. J. Autism Dev. Disord. 44, 521–531 (2014).
pubmed: 23888359 doi: 10.1007/s10803-013-1899-3
Guastella, A. J. et al. Recommendations for the standardisation of oxytocin nasal administration and guidelines for its reporting in human research. Psychoneuroendocrinology 38, 612–625 (2013).
pubmed: 23265311 doi: 10.1016/j.psyneuen.2012.11.019
Luis Royo, J., Hidalgo, M. & Ruiz, A. Pyrosequencing protocol using a universal biotinylated primer for mutation detection and SNP genotyping. Nat. Protoc. 2, 1734–1739 (2007).
doi: 10.1038/nprot.2007.244
Krol, K. M., Puglia, M. H., Morris, J. P., Connelly, J. J. & Grossmann, T. Epigenetic modification of the oxytocin receptor gene is associated with emotion processing in the infant brain. Dev. Cogn. Neurosci. 37, 1–8 (2019).
doi: 10.1016/j.dcn.2019.100648
Alaerts, K., Bernaerts, S., Vanaudenaerde, B., Daniels, N. & Wenderoth, N. Amygdala–Hippocampal Connectivity Is Associated With Endogenous Levels of Oxytocin and Can Be Altered by Exogenously Administered Oxytocin in Adults With Autism. Biol. Psychiatry Cogn. Neurosci. Neuroimag 4, 655–663 (2019).
Bodfish, J. W., Symons, F. J., Parker, D. E. & Lewis, M. H. Varieties of repetitive behaviour in autism: comparison to mental retardation. J. Autism Dev. Disord. 30, 237–243 (2000).
pubmed: 11055459 doi: 10.1023/A:1005596502855
Muris, P., Bodden, D., Hale, W., Birmaher, B. & Mayer, B. SCARED-NL. Vragenlijst over angst en bang-zijn bij kinderen en adolescenten. Handleiding bij de gereviseerde Nederlandse versie van de Screen for Child Anxiety Related Emotional Disorders. Preprint at (2007).
Finzi, R., Cohen, O., Sapir, Y. & Weizman, A. Attachment Styles in Maltreated Children: A Comparative Study. Child Psychiatry Hum. Dev. 31, 113–128 (2000).
pubmed: 11089300 doi: 10.1023/A:1001944509409

Auteurs

Matthijs Moerkerke (M)

Center for Developmental Psychiatry, Department of Neurosciences, KU Leuven, Leuven, Belgium.
Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.

Nicky Daniels (N)

Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.
Research Group for Neurorehabilitation, Department of Rehabilitation Sciences, KU Leuven, Leuven, Belgium.

Laura Tibermont (L)

Center for Developmental Psychiatry, Department of Neurosciences, KU Leuven, Leuven, Belgium.
Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.

Tiffany Tang (T)

Center for Developmental Psychiatry, Department of Neurosciences, KU Leuven, Leuven, Belgium.
Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.

Margaux Evenepoel (M)

Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.
Research Group for Neurorehabilitation, Department of Rehabilitation Sciences, KU Leuven, Leuven, Belgium.

Stephanie Van der Donck (S)

Center for Developmental Psychiatry, Department of Neurosciences, KU Leuven, Leuven, Belgium.
Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.

Edward Debbaut (E)

Center for Developmental Psychiatry, Department of Neurosciences, KU Leuven, Leuven, Belgium.
Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.

Jellina Prinsen (J)

Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.
Research Group for Neurorehabilitation, Department of Rehabilitation Sciences, KU Leuven, Leuven, Belgium.

Viktoria Chubar (V)

University Psychiatric Centre, KU Leuven, Leuven, Belgium.

Stephan Claes (S)

University Psychiatric Centre, KU Leuven, Leuven, Belgium.

Bart Vanaudenaerde (B)

Laboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Illness and Metabolism, KU Leuven, Leuven, Belgium.

Lynn Willems (L)

Laboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Illness and Metabolism, KU Leuven, Leuven, Belgium.

Jean Steyaert (J)

Center for Developmental Psychiatry, Department of Neurosciences, KU Leuven, Leuven, Belgium.
Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.

Bart Boets (B)

Center for Developmental Psychiatry, Department of Neurosciences, KU Leuven, Leuven, Belgium.
Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium.

Kaat Alaerts (K)

Leuven Autism Research (LAuRes), KU Leuven, Leuven, Belgium. kaat.alaerts@kuleuven.be.
Research Group for Neurorehabilitation, Department of Rehabilitation Sciences, KU Leuven, Leuven, Belgium. kaat.alaerts@kuleuven.be.

Classifications MeSH