IL-12 reprograms CAR-expressing natural killer T cells to long-lived Th1-polarized cells with potent antitumor activity.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
02 Jan 2024
Historique:
received: 28 07 2023
accepted: 07 12 2023
medline: 4 1 2024
pubmed: 4 1 2024
entrez: 3 1 2024
Statut: epublish

Résumé

Human natural killer T cells (NKTs) are innate-like T lymphocytes increasingly used for cancer immunotherapy. Here we show that human NKTs expressing the pro-inflammatory cytokine interleukin-12 (IL-12) undergo extensive and sustained molecular and functional reprogramming. Specifically, IL-12 instructs and maintains a Th1-polarization program in NKTs in vivo without causing their functional exhaustion. Furthermore, using CD62L as a marker of memory cells in human NKTs, we observe that IL-12 maintains long-term CD62L-expressing memory NKTs in vivo. Notably, IL-12 initiates a de novo programming of memory NKTs in CD62L-negative NKTs indicating that human NKTs circulating in the peripheral blood possess an intrinsic differentiation hierarchy, and that IL-12 plays a role in promoting their differentiation to long-lived Th1-polarized memory cells. Human NKTs engineered to co-express a Chimeric Antigen Receptor (CAR) coupled with the expression of IL-12 show enhanced antitumor activity in leukemia and neuroblastoma tumor models, persist long-term in vivo and conserve the molecular signature driven by the IL-12 expression. Thus IL-12 reveals an intrinsic plasticity of peripheral human NKTs that may play a crucial role in the development of cell therapeutics.

Identifiants

pubmed: 38167707
doi: 10.1038/s41467-023-44310-y
pii: 10.1038/s41467-023-44310-y
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

89

Subventions

Organisme : NIDDK NIH HHS
ID : P30 DK034987
Pays : United States

Informations de copyright

© 2024. The Author(s).

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Auteurs

Elisa Landoni (E)

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.

Mark G Woodcock (MG)

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.
Division of Oncology, Department of Medicine, University of North Carolina, Chapel Hill, NC, USA.

Gabriel Barragan (G)

Center for Advanced Innate Cell Therapy, Texas Children's Cancer Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.

Gabriele Casirati (G)

Dana-Farber/Boston Children's Cancer and Blood Disorder Center, Boston, USA.
Harvard Medical School, Boston, USA.

Vincenzo Cinella (V)

Dana-Farber/Boston Children's Cancer and Blood Disorder Center, Boston, USA.
Harvard Medical School, Boston, USA.

Simone Stucchi (S)

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.

Leah M Flick (LM)

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.

Tracy A Withers (TA)

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.

Hanna Hudson (H)

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.

Giulia Casorati (G)

Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.

Paolo Dellabona (P)

Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.

Pietro Genovese (P)

Dana-Farber/Boston Children's Cancer and Blood Disorder Center, Boston, USA.
Harvard Medical School, Boston, USA.

Barbara Savoldo (B)

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.
Department of Pediatrics, University of North Carolina, Chapel Hill, NC, USA.

Leonid S Metelitsa (LS)

Center for Advanced Innate Cell Therapy, Texas Children's Cancer Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.

Gianpietro Dotti (G)

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA. gdotti@med.unc.edu.
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC, USA. gdotti@med.unc.edu.

Classifications MeSH