Rapid Visual Detection of Spiroplasma eriocheiris by Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye.
Hydroxy naphthol blue dye
Loop-mediated isothermal amplification
Spiroplasma eriocheiris
Visual detection
Journal
Marine biotechnology (New York, N.Y.)
ISSN: 1436-2236
Titre abrégé: Mar Biotechnol (NY)
Pays: United States
ID NLM: 100892712
Informations de publication
Date de publication:
03 Jan 2024
03 Jan 2024
Historique:
received:
13
10
2023
accepted:
22
12
2023
medline:
4
1
2024
pubmed:
4
1
2024
entrez:
3
1
2024
Statut:
aheadofprint
Résumé
In recent years, a new type of Spiroplasma has been found that can cause "tremor disease" of the Chinese mitten crab Eriocheir sinensis. The outbreak of epidemic tremor disease has caused a serious setback in the Chinese mitten crab farming industry, with an incidence rate of more than 30% and mortality rates of 80-100%. Therefore, finding a sensitive method to detect tremor disease in E. sinensis has become a current research focus. In this research, a loop-mediated isothermal amplification detection method coupled with hydroxynaphthol blue dye (LAMP-HNB) was developed and used to rapidly detect Spiroplasma eriocheiris. First, we designed and synthesized specific outer primers, inner primers and loop primers based on the 16S ribosomal RNA gene of S. eriocheiris. Second, the LAMP-HNB detection method for S. eriocheiris was successfully established by screening the primers, adjusting the temperature and time of the reaction, and optimizing the concentrations of Mg
Identifiants
pubmed: 38170295
doi: 10.1007/s10126-023-10282-9
pii: 10.1007/s10126-023-10282-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fish Innovation Team of Shandong Agriculture Research System
ID : SDAIT-12-06
Organisme : the Advanced Talents Foundation of QAU
ID : 6651118016
Organisme : Qingdao Agricultural University Doctoral Start-Up Fund
ID : 6631122030
Organisme : National Natural Science Foundation of China
ID : 32002421
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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