Selection and Characterization of DNA Aptamers for Cytidine and Uridine.

Aptamer Biosensor Capture-SELEX Cytidine Uridine

Journal

Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360

Informations de publication

Date de publication:
05 Jan 2024
Historique:
revised: 30 11 2023
received: 25 09 2023
medline: 5 1 2024
pubmed: 5 1 2024
entrez: 5 1 2024
Statut: aheadofprint

Résumé

Cytidine and uridine are two essential pyrimidine ribonucleotides, and accurate detection of these nucleosides holds significant biological importance. While many aptamers were reported to bind purines, little success was achieved for pyrimidine binding. This study employs the library-immobilization capture-SELEX technique to isolate aptamers capable of selectively binding to cytidine and uridine. First, a selection was performed using a mixture of cytidine and uridine as the target. This selection led to the isolation of a highly selective aptamer for cytidine with a dissociation constant (K

Identifiants

pubmed: 38180305
doi: 10.1002/cbic.202300656
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202300656

Subventions

Organisme : Natural Sciences and Engineering Research Council of Canada (NSERC)
Organisme : China Scholarship Council (CSC)

Informations de copyright

© 2024 The Authors. ChemBioChem published by Wiley-VCH GmbH.

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Auteurs

Lide Gu (L)

State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian, 361102, China.
Department of Chemistry, Waterloo Institute for Nanotechnology University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.

Jiajie Zheng (J)

Department of Chemistry, Waterloo Institute for Nanotechnology University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.

Yao Zhang (Y)

State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian, 361102, China.

Deli Wang (D)

State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian, 361102, China.

Juewen Liu (J)

Department of Chemistry, Waterloo Institute for Nanotechnology University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.

Classifications MeSH