Cultivation of Schwann cells from fresh and non-fresh adult equine peripheral nerves.

CD271 CD90 Peripheral nerve Primary Schwann cells horse neuropathy

Journal

Journal of neuroscience methods
ISSN: 1872-678X
Titre abrégé: J Neurosci Methods
Pays: Netherlands
ID NLM: 7905558

Informations de publication

Date de publication:
03 Jan 2024
Historique:
received: 19 09 2023
revised: 12 12 2023
accepted: 31 12 2023
medline: 6 1 2024
pubmed: 6 1 2024
entrez: 5 1 2024
Statut: aheadofprint

Résumé

Over the past 25 years, acquired equine polyneuropathy (AEP) has emerged as a neurological disease in Scandinavian horses. This condition is characterized by histopathological features including the presence of Schwann cell (SC) inclusions. Cultivated equine SCs would serve as a valuable resource for investigations of factors triggering this Schwannopathy. Ideally, cells should be sampled for cultivation from fresh nerves immediately after death of the animal, however the availability of fresh material is limited, due to the inconsistent case load and the inherent technical and practical challenges to collection of samples in the field. This study aimed to cultivate SCs from adult equine peripheral nerves and assess their ability to survive in sampled nerve material over time to simulate harvesting of SCs in field situations. Peripheral nerves from five non-neurological horses were used. After euthanasia, both fresh and non-fresh nerve samples were harvested from each horse. Flow cytometry was employed to confirm the cellular identity and to determine the SC purity. The results revealed successful establishment of SC cultures from adult equine peripheral nerves, with the potential to achieve high SC purity from both fresh and non-fresh nerve samples. While most SC isolation methods focus on harvest of cells from fresh nerve materials from laboratory animals, our approach highlights the possibility of utilizing SC cultures from field-harvested and transported nerve samples from horses. We describe a method for isolating SCs with high purity from both fresh and non-fresh peripheral nerves of adult horses.

Sections du résumé

BACKGROUND BACKGROUND
Over the past 25 years, acquired equine polyneuropathy (AEP) has emerged as a neurological disease in Scandinavian horses. This condition is characterized by histopathological features including the presence of Schwann cell (SC) inclusions. Cultivated equine SCs would serve as a valuable resource for investigations of factors triggering this Schwannopathy. Ideally, cells should be sampled for cultivation from fresh nerves immediately after death of the animal, however the availability of fresh material is limited, due to the inconsistent case load and the inherent technical and practical challenges to collection of samples in the field. This study aimed to cultivate SCs from adult equine peripheral nerves and assess their ability to survive in sampled nerve material over time to simulate harvesting of SCs in field situations.
NEW METHODS METHODS
Peripheral nerves from five non-neurological horses were used. After euthanasia, both fresh and non-fresh nerve samples were harvested from each horse. Flow cytometry was employed to confirm the cellular identity and to determine the SC purity.
RESULTS RESULTS
The results revealed successful establishment of SC cultures from adult equine peripheral nerves, with the potential to achieve high SC purity from both fresh and non-fresh nerve samples.
COMPARISON WITH EXISTING METHOD CONCLUSIONS
While most SC isolation methods focus on harvest of cells from fresh nerve materials from laboratory animals, our approach highlights the possibility of utilizing SC cultures from field-harvested and transported nerve samples from horses.
CONCLUSIONS CONCLUSIONS
We describe a method for isolating SCs with high purity from both fresh and non-fresh peripheral nerves of adult horses.

Identifiants

DOI: 10.1016/j.jneumeth.2023.110054 PMID: 38181868
pubmed: 38181868
pii: S0165-0270(23)00273-X
doi: 10.1016/j.jneumeth.2023.110054
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110054

Informations de copyright

Copyright © 2024. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Siv Hanche-Olsen reports was provided by Swedish-Norwegian Foundation for Equine Research, supportet by the Reseach Concil of Norway Competing interests The authors declare that they have no competing interests.

Auteurs

Elise Friis Kvigstad (EF)

Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway.

Ingvild Ketilsdotter Øverland (IK)

Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway.

Fredrik Strebel Skedsmo (FS)

Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway.

Karin Hultin Jäderlund (KH)

Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oluf Thesensvei 24/30, 1433 Ås, Norway.

Gittan Gröndahl (G)

Department of Animal Health and Microbial Strategies, National Veterinary Institute, 75189 Uppsala, Sweden.

Siv Hanche-Olsen (S)

Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oluf Thesensvei 24/30, 1433 Ås, Norway.

Gjermund Gunnes (G)

Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, 1433 Ås, Norway. Electronic address: gjermund.gunnes@nmbu.no.

Classifications MeSH