Highly efficient biosynthesis of salidroside by a UDP-glucosyltransferase-catalyzed cascade reaction.

Salidroside Sucrose synthase Tyrosol UDP-glucose regeneration UDP-glucosyltransferase

Journal

Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051

Informations de publication

Date de publication:
06 Jan 2024
Historique:
received: 25 01 2023
accepted: 19 11 2023
revised: 23 10 2023
medline: 7 1 2024
pubmed: 7 1 2024
entrez: 6 1 2024
Statut: aheadofprint

Résumé

Salidroside is an important plant-derived aromatic compound with diverse biological properties. The main objective of this study was to synthesize salidroside from tyrosol using UDP-glucosyltransferase (UGT) with in situ regeneration of UDP-glucose (UDPG). The UDP-glucosyltransferase 85A1 (UGT85A1) from Arabidopsis thaliana, which showed high activity and regioselectivity towards tyrosol, was selected for the production of salidroside. Then, an in vitro cascade reaction for in situ regeneration of UDPG was constructed by coupling UGT85A1 to sucrose synthase from Glycine max (GmSuSy). The optimal UGT85A1-GmSuSy activity ratio of 1:2 was determined to balance the efficiency of salidroside production and UDP-glucose regeneration. Different cascade reaction conditions for salidroside production were also determined. Under the optimized condition, salidroside was produced at a titer of 6.0 g/L with a corresponding molar conversion of 99.6% and a specific productivity of 199.1 mg/L/h in a continuous feeding reactor. This is the highest salidroside titer ever reported so far using biocatalytic approach.

Identifiants

DOI: 10.1007/s10529-023-03453-0 PMID: 38184486
pubmed: 38184486
doi: 10.1007/s10529-023-03453-0
pii: 10.1007/s10529-023-03453-0
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Open Fund of Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources
ID : TCMRPSU-2022-03
Organisme : Natural Science Foundation of Higher Education Institutions of Anhui Province
ID : 2023AH052649
Organisme : Fund of Generic Technology Research center for Anhui Traditional Chinese Medicine Industry
ID : AHTCMGTRC-2023-09
Organisme : National Natural Science Foundation of China
ID : 32201979
Organisme : University Research Project of Anhui Province
ID : 2022AH051682

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer Nature B.V.

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Auteurs

Guosi Li (G)

Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China. liguosi1989@163.com.
Anhui Engineering Research Center for Eco-Agriculture of Traditional Chinese Medicine, Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012, Anhui, China. liguosi1989@163.com.
ORCID: 0000-0003-4677-2666

Qilin Xu (Q)

Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China.
Anhui Engineering Research Center for Eco-Agriculture of Traditional Chinese Medicine, Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012, Anhui, China.

Nan Hu (N)

Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China.

Xinyang Liu (X)

Anhui Engineering Research Center for Eco-Agriculture of Traditional Chinese Medicine, Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012, Anhui, China.

Yiqi Jiang (Y)

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, Zhejiang, China.

Hailong Xue (H)

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, Zhejiang, China.

Yongjun Zang (Y)

Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China.
Anhui Engineering Research Center for Eco-Agriculture of Traditional Chinese Medicine, Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012, Anhui, China.

Fucheng Zhu (F)

Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, West Anhui University, Lu'an, 237012, Anhui, China. fucheng323@163.com.
Anhui Engineering Research Center for Eco-Agriculture of Traditional Chinese Medicine, Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012, Anhui, China. fucheng323@163.com.

Classifications MeSH