The cellular landscape of the endochondral bone during the transition to extrauterine life.
birth
endochondral ossification
fibroblastmesenchymal stromal skeletal progenitors
mouse
scRNA-seq
Journal
Immunology and cell biology
ISSN: 1440-1711
Titre abrégé: Immunol Cell Biol
Pays: United States
ID NLM: 8706300
Informations de publication
Date de publication:
07 Jan 2024
07 Jan 2024
Historique:
revised:
09
12
2023
received:
22
11
2023
accepted:
12
12
2023
medline:
7
1
2024
pubmed:
7
1
2024
entrez:
7
1
2024
Statut:
aheadofprint
Résumé
The cellular complexity of the endochondral bone underlies its essential and pleiotropic roles during organismal life. While the adult bone has received significant attention, we still lack a deep understanding of the perinatal bone cellulome. Here, we have profiled the full composition of the murine endochondral bone at the single-cell level during the transition from fetal to newborn life and in comparison with the adult tissue, with particular emphasis on the mesenchymal compartment. The perinatal bone contains different fibroblastic clusters with blastema-like characteristics in organizing and supporting skeletogenesis, angiogenesis and hematopoiesis. Our data also suggest dynamic inter- and intra-compartment interactions, as well as a bone marrow milieu that seems prone to anti-inflammation, which we hypothesize is necessary to ensure the proper program of lymphopoiesis and the establishment of central and peripheral tolerance in early life. Our study provides an integrative roadmap for the future design of genetic and cellular functional assays to validate cellular interactions and lineage relationships within the perinatal bone.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Consejería de Conocimiento, Investigación y Universidad, Junta de Andalucía
ID : PY20-00421
Organisme : Ministerio de Ciencia e Innovación
ID : CEX2020-001088-M
Informations de copyright
© 2024 The Authors. Immunology & Cell Biology published by John Wiley & Sons Australia, Ltd on behalf of the Australian and New Zealand Society for Immunology, Inc.
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