Biochemical and thermodynamic properties of de novo synthesized urease from Vicia sativa seeds with enhanced industrial applications.

Kinetic constant Thermostability Urease

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
05 Jan 2024
Historique:
received: 08 11 2023
revised: 14 12 2023
accepted: 31 12 2023
medline: 8 1 2024
pubmed: 8 1 2024
entrez: 7 1 2024
Statut: aheadofprint

Résumé

Urease is one of the most significant enzymes in the industry. The objective of this research was to isolate and partially purify urease from Vicia sativa seeds with urease characterization. With a 6.4 % yield, the purification fold was 9.0. By using chromatography, it was determined that the isolated urease had a molecular weight of 55 kDa. The maximum urease activity was found following a 60-s incubation period at 40 °C and pH 8. The activity of urease was significantly boosted by a mean of calcium, barium, DL-dithiothreitol, Na

Identifiants

DOI: 10.1016/j.ijbiomac.2023.129190 PMID: 38185304
pubmed: 38185304
pii: S0141-8130(23)06089-0
doi: 10.1016/j.ijbiomac.2023.129190
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

129190

Informations de copyright

Copyright © 2023. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest The author has no conflict of interest exits in the submission of this manuscript and the authors have complied with journal ethical requirements. Moreover, manuscript is approved by all authors as well as all the authorities acknowledged for publication.

Auteurs

Amal M Sindi (AM)

Department of Oral Diagnostic Sciences, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.

Umber Zaman (U)

Institute of Chemical Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan.

Ebraheem Abdu Musad Saleh (EAM)

Department of Chemistry, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.

Asmaa F Kassem (AF)

Department of Chemistry, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Cairo 12622, Egypt.

Khalil Ur Rahman (KU)

Institute of Chemical Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan. Electronic address: rehmankhalil025@gmail.com.

Shahid Ullah Khan (SU)

Integrative Science Centre of Germplasm Creation in Western China (CHONGQING) Science City and Southwest University, College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China; Department of Biochemistry, Women Medical and Dental College, Khyber Medical University KP, Pakistan.

Majed Alharbi (M)

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.

Waleed Y Rizg (WY)

Center of Innovation in Personalized Medicine (CIPM), 3D Bioprinting Unit, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Khaled Mohamed Omar (KM)

Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Mohammed Ali A Majrashi (MAA)

Department of Pharmacology, College of Medicine, University of Jeddah, Jeddah 23890, Saudi Arabia.

Awaji Y Safhi (AY)

Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia.

Ehab A Abdelrahman (EA)

Department of Chemistry, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia; Chemistry Department, Faculty of Science, Benha University, Benha 13518, Egypt.

Classifications MeSH