Large-scale cultivation of human iPS cells in bioreactor with reciprocal mixing.
Bioreactor
Human iPS cells
Reciprocal mixing
Shear stress
Suspension culture
Journal
Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800
Informations de publication
Date de publication:
06 Jan 2024
06 Jan 2024
Historique:
received:
13
09
2023
revised:
28
11
2023
accepted:
12
12
2023
medline:
8
1
2024
pubmed:
8
1
2024
entrez:
7
1
2024
Statut:
aheadofprint
Résumé
A substantial number of human iPS cells (hiPSCs) is needed for cell therapy to be successful against various diseases. We previously reported on a bioreactor with reciprocal mixing that produces specific physical properties that differ from those of conventional bioreactors with rotary paddle stirring. Moreover, such reactors not only provide a homogeneous environment but also allow the control of spheroid size by changing the mixing speed. In this study, we applied this bioreactor to the large-scale cultivation of hiPSCs. Approximately 10 billion hiPSCs were obtained from 2.0 L of culture, and the high expression of pluripotency markers was maintained. Our findings indicate that a bioreactor with reciprocal mixing can be used for large-scale hiPSC cultivation.
Identifiants
pubmed: 38185598
pii: S1389-1723(23)00372-9
doi: 10.1016/j.jbiosc.2023.12.008
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2023 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.