Interaction modes of human orexin 2 receptor with selective and nonselective antagonists studied by NMR spectroscopy.

(13)C methyl methionine NMR EMPA GPCR OX2R orexin 2 receptor structural plasticity suvorexant

Journal

Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697

Informations de publication

Date de publication:
01 Jan 2024
Historique:
received: 24 02 2023
revised: 17 08 2023
accepted: 13 12 2023
medline: 10 1 2024
pubmed: 10 1 2024
entrez: 9 1 2024
Statut: aheadofprint

Résumé

Orexin neuropeptides have many physiological roles in the sleep-wake cycle, feeding behavior, reward demands, and stress responses by activating cognitive receptors, the orexin receptors (OX1R and OX2R), distributed in the brain. There are only subtle differences between OX1R and OX2R in the orthosteric site, which has hindered the rational development of subtype-selective antagonists. In this study, we utilized solution-state NMR to capture the structural plasticity of OX2R labeled with

Identifiants

DOI: 10.1016/j.str.2023.12.008 PMID: 38194963
pubmed: 38194963
pii: S0969-2126(23)00449-5
doi: 10.1016/j.str.2023.12.008
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no conflict of interest.

Auteurs

Kayo Imamura (K)

Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

Ken-Ichi Akagi (KI)

Section of Laboratory Equipment, National Institute of Biomedical Innovation, Health, and Nutrition, Osaka 567-0085, Japan.

Yohei Miyanoiri (Y)

Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

Hirokazu Tsujimoto (H)

Department of Cell Biology and Medical Chemistry, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.

Takatsugu Hirokawa (T)

Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan; Transborder Medical Research Center, University of Tsukuba, Tsukuba 305-8575, Japan.

Hideo Ashida (H)

Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

Kaori Murakami (K)

Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

Asuka Inoue (A)

Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980-8578, Japan.

Ryoji Suno (R)

Department of Medical Chemistry, Kansai Medical University, Hirakata 573-1010, Japan.

Takahisa Ikegami (T)

Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehiro, Yokohama, Kanagawa 230-0045, Japan.

Naotaka Sekiyama (N)

Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.

So Iwata (S)

Department of Cell Biology and Medical Chemistry, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.

Takuya Kobayashi (T)

Department of Medical Chemistry, Kansai Medical University, Hirakata 573-1010, Japan.

Hidehito Tochio (H)

Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: tochio@mb.biophys.kyoto-u.ac.jp.

Classifications MeSH