EMID2 is a novel biotherapeutic for aggressive cancers identified by in vivo screening.

AAV vectors Biotherapeutics Cancer Cell invasiveness Gene therapy In vivo screening

Journal

Journal of experimental & clinical cancer research : CR
ISSN: 1756-9966
Titre abrégé: J Exp Clin Cancer Res
Pays: England
ID NLM: 8308647

Informations de publication

Date de publication:
10 Jan 2024
Historique:
received: 30 09 2023
accepted: 22 12 2023
medline: 10 1 2024
pubmed: 10 1 2024
entrez: 9 1 2024
Statut: epublish

Résumé

New drugs to tackle the next pathway or mutation fueling cancer are constantly proposed, but 97% of them are doomed to fail in clinical trials, largely because they are identified by cellular or in silico screens that cannot predict their in vivo effect. We screened an Adeno-Associated Vector secretome library (> 1000 clones) directly in vivo in a mouse model of cancer and validated the therapeutic effect of the first hit, EMID2, in both orthotopic and genetic models of lung and pancreatic cancer. EMID2 overexpression inhibited both tumor growth and metastatic dissemination, consistent with prolonged survival of patients with high levels of EMID2 expression in the most aggressive human cancers. Mechanistically, EMID2 inhibited TGFβ maturation and activation of cancer-associated fibroblasts, resulting in more elastic ECM and reduced levels of YAP in the nuclei of cancer cells. This is the first in vivo screening, precisely designed to identify proteins able to interfere with cancer cell invasiveness. EMID2 was selected as the most potent protein, in line with the emerging relevance of the tumor extracellular matrix in controlling cancer cell invasiveness and dissemination, which kills most of cancer patients.

Sections du résumé

BACKGROUND BACKGROUND
New drugs to tackle the next pathway or mutation fueling cancer are constantly proposed, but 97% of them are doomed to fail in clinical trials, largely because they are identified by cellular or in silico screens that cannot predict their in vivo effect.
METHODS METHODS
We screened an Adeno-Associated Vector secretome library (> 1000 clones) directly in vivo in a mouse model of cancer and validated the therapeutic effect of the first hit, EMID2, in both orthotopic and genetic models of lung and pancreatic cancer.
RESULTS RESULTS
EMID2 overexpression inhibited both tumor growth and metastatic dissemination, consistent with prolonged survival of patients with high levels of EMID2 expression in the most aggressive human cancers. Mechanistically, EMID2 inhibited TGFβ maturation and activation of cancer-associated fibroblasts, resulting in more elastic ECM and reduced levels of YAP in the nuclei of cancer cells.
CONCLUSION CONCLUSIONS
This is the first in vivo screening, precisely designed to identify proteins able to interfere with cancer cell invasiveness. EMID2 was selected as the most potent protein, in line with the emerging relevance of the tumor extracellular matrix in controlling cancer cell invasiveness and dissemination, which kills most of cancer patients.

Identifiants

pubmed: 38195652
doi: 10.1186/s13046-023-02942-4
pii: 10.1186/s13046-023-02942-4
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

15

Subventions

Organisme : Fondazione AIRC per la ricerca sul cancro ETS
ID : 24529

Informations de copyright

© 2024. The Author(s).

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Auteurs

Ambra Cappelletto (A)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Edoardo Alfì (E)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
Department of Life Sciences, University of Trieste, Trieste, Italy.

Nina Volf (N)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Thi Van Anh Vu (TVA)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Francesca Bortolotti (F)

Molecular Medicine, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Giulio Ciucci (G)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Simone Vodret (S)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Marco Fantuz (M)

Veneto Institute of Molecular Medicine, Padova, Italy.
University of Padova, Padova, Italy.

Martina Perin (M)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Andrea Colliva (A)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Giacomo Rozzi (G)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Matilde Rossi (M)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Giulia Ruozi (G)

Molecular Medicine, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Lorena Zentilin (L)

Molecular Medicine, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Roman Vuerich (R)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
Department of Life Sciences, University of Trieste, Trieste, Italy.

Daniele Borin (D)

Department of Engineering and Architecture, University of Trieste, Trieste, Italy.

Romano Lapasin (R)

Department of Engineering and Architecture, University of Trieste, Trieste, Italy.

Silvano Piazza (S)

Bioinformatics, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
Bioinformatics Facility, Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, Trento, Italy.

Mattia Chiesa (M)

Centro Cardiologico Monzino, Milano, Italy.

Daniela Lorizio (D)

Centro Cardiologico Monzino, Milano, Italy.

Luca Triboli (L)

Department of Life Sciences, University of Trieste, Trieste, Italy.
Cancer Cell Signaling, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Sandeep Kumar (S)

Wallace H. Coulter Department of Biomedical Engineering, Emory University, Georgia Institute of Technology, Atlanta, GA, USA.

Gaia Morello (G)

Tumor Immunology Unit, Department of Sciences for Health Promotion and Mother-Child Care "G. D'Alessandro", University of Palermo, Palermo, Italy.

Claudio Tripodo (C)

Tumor Immunology Unit, Department of Sciences for Health Promotion and Mother-Child Care "G. D'Alessandro", University of Palermo, Palermo, Italy.
Histopathology Unit, Institute of Molecular Oncology Foundation (IFOM), ETS - The AIRC Institute of Molecular Oncology, Milan, Italy.

Maurizio Pinamonti (M)

Pathology Department Azienda Sanitaria Universitaria Giuliano-Isontina and University of Trieste, Trieste, Italy.

Giulia Maria Piperno (GM)

Cellular Immunology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Federica Benvenuti (F)

Cellular Immunology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Alessandra Rustighi (A)

Department of Life Sciences, University of Trieste, Trieste, Italy.
Cancer Cell Signaling, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

Hanjoong Jo (H)

Wallace H. Coulter Department of Biomedical Engineering, Emory University, Georgia Institute of Technology, Atlanta, GA, USA.

Stefano Piccolo (S)

University of Padova, Padova, Italy.

Giannino Del Sal (G)

Department of Life Sciences, University of Trieste, Trieste, Italy.
Cancer Cell Signaling, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
IFOM ETS, The AIRC Institute of Molecular Oncology, Milan, Italy.

Alessandro Carrer (A)

Veneto Institute of Molecular Medicine, Padova, Italy.
University of Padova, Padova, Italy.

Mauro Giacca (M)

Molecular Medicine, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
King's College London, British Heart Foundation Centre of Research Excellence, London, UK.
Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.

Serena Zacchigna (S)

Cardiovascular Biology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy. zacchign@icgeb.org.
Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy. zacchign@icgeb.org.

Classifications MeSH