Direct detection of canine picornavirus complete coding sequence in wastewater using long-range reverse-transcriptase polymerase chain reaction and long-read sequencing.
Canine picornavirus
Long-range RT-PCR
Long-read sequencing
Picornaviridae
Wastewater-based epidemiology
Journal
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
ISSN: 1567-7257
Titre abrégé: Infect Genet Evol
Pays: Netherlands
ID NLM: 101084138
Informations de publication
Date de publication:
08 Jan 2024
08 Jan 2024
Historique:
received:
24
10
2023
revised:
03
01
2024
accepted:
06
01
2024
medline:
11
1
2024
pubmed:
11
1
2024
entrez:
10
1
2024
Statut:
aheadofprint
Résumé
We describe four complete coding sequence (cCDS) of canine picornavirus from wastewater in Arizona, USA detected by coupling cCDS single-contig (~7.5 kb) reverse-transcriptase polymerase chain reaction (RT-PCR) and low-cost long-read high-throughput sequencing. For viruses of medical/veterinary importance, this workflow expands possibilities of wastewater based genomic epidemiology for exploring virus evolutionary dynamics especially in low-resource settings.
Identifiants
pubmed: 38199505
pii: S1567-1348(24)00001-7
doi: 10.1016/j.meegid.2024.105550
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105550Informations de copyright
Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: RUH and EMD are co-founders of AquaVitas, LLC, Phoenix, Arizona, United States, an Arizona State University startup company providing commercial services in wastewater-based epidemiology. RUH also is the founder of OneWaterOneHealth, a nonprofit project of the Arizona State University Foundation.