Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
11 Jan 2024
11 Jan 2024
Historique:
received:
29
11
2022
accepted:
19
12
2023
medline:
12
1
2024
pubmed:
12
1
2024
entrez:
11
1
2024
Statut:
epublish
Résumé
Complex II (CII) activity controls phenomena that require crosstalk between metabolism and signaling, including neurodegeneration, cancer metabolism, immune activation, and ischemia-reperfusion injury. CII activity can be regulated at the level of assembly, a process that leverages metastable assembly intermediates. The nature of these intermediates and how CII subunits transfer between metastable complexes remains unclear. In this work, we identify metastable species containing the SDHA subunit and its assembly factors, and we assign a preferred temporal sequence of appearance of these species during CII assembly. Structures of two species show that the assembly factors undergo disordered-to-ordered transitions without the appearance of significant secondary structure. The findings identify that intrinsically disordered regions are critical in regulating CII assembly, an observation that has implications for the control of assembly in other biomolecular complexes.
Identifiants
pubmed: 38212624
doi: 10.1038/s41467-023-44563-7
pii: 10.1038/s41467-023-44563-7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
473Subventions
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : GM61606
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : GM61606
Organisme : American Heart Association (American Heart Association, Inc.)
ID : 19POST34450093
Organisme : U.S. Department of Veterans Affairs (Department of Veterans Affairs)
ID : IK6BX004215
Informations de copyright
© 2024. The Author(s).
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