Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
11 Jan 2024
Historique:
received: 29 11 2022
accepted: 19 12 2023
medline: 12 1 2024
pubmed: 12 1 2024
entrez: 11 1 2024
Statut: epublish

Résumé

Complex II (CII) activity controls phenomena that require crosstalk between metabolism and signaling, including neurodegeneration, cancer metabolism, immune activation, and ischemia-reperfusion injury. CII activity can be regulated at the level of assembly, a process that leverages metastable assembly intermediates. The nature of these intermediates and how CII subunits transfer between metastable complexes remains unclear. In this work, we identify metastable species containing the SDHA subunit and its assembly factors, and we assign a preferred temporal sequence of appearance of these species during CII assembly. Structures of two species show that the assembly factors undergo disordered-to-ordered transitions without the appearance of significant secondary structure. The findings identify that intrinsically disordered regions are critical in regulating CII assembly, an observation that has implications for the control of assembly in other biomolecular complexes.

Identifiants

pubmed: 38212624
doi: 10.1038/s41467-023-44563-7
pii: 10.1038/s41467-023-44563-7
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

473

Subventions

Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : GM61606
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : GM61606
Organisme : American Heart Association (American Heart Association, Inc.)
ID : 19POST34450093
Organisme : U.S. Department of Veterans Affairs (Department of Veterans Affairs)
ID : IK6BX004215

Informations de copyright

© 2024. The Author(s).

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Auteurs

Pankaj Sharma (P)

Department of Pharmacology, Vanderbilt University, Nashville, TN, 37232, USA.

Elena Maklashina (E)

Molecular Biology Division, San Francisco VA Health Care System, San Francisco, CA, 94121, USA.
Department of Biochemistry & Biophysics, University of California, San Francisco, CA, 94158, USA.

Markus Voehler (M)

Department of Chemistry Vanderbilt University, Nashville, TN, 37232, USA.
Center for Structural Biology Vanderbilt University, Nashville, TN, 37232, USA.

Sona Balintova (S)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic.
Faculty of Science, Charles University, 128 00, Prague 2, Czech Republic.

Sarka Dvorakova (S)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic.

Michal Kraus (M)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic.

Katerina Hadrava Vanova (KH)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic.
Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20814, USA.

Zuzana Nahacka (Z)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic.

Renata Zobalova (R)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic.

Stepana Boukalova (S)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic.

Kristyna Cunatova (K)

Institute of Physiology, Czech Academy of Sciences, Prague 4, 142 20, Prague, Czech Republic.

Tomas Mracek (T)

Institute of Physiology, Czech Academy of Sciences, Prague 4, 142 20, Prague, Czech Republic.

Hans K Ghayee (HK)

Department of Medicine, Division of Endocrinology & Metabolism, University of Florida College of Medicine and Malcom Randall, VA Medical Center, Gainesville, FL, 32608, USA.

Karel Pacak (K)

Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20814, USA.

Jakub Rohlena (J)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic.

Jiri Neuzil (J)

Institute of Biotechnology, Czech Academy of Sciences, 252 50, Prague-West, Czech Republic. j.neuzil@griffith.edu.au.
Faculty of Science, Charles University, 128 00, Prague 2, Czech Republic. j.neuzil@griffith.edu.au.
School of Pharmacy and Medical Science, Griffith University, Southport, QLD, 4222, Australia. j.neuzil@griffith.edu.au.
1st Faculty of Medicine, Charles University, 128 00, Prague 2, Czech Republic. j.neuzil@griffith.edu.au.

Gary Cecchini (G)

Molecular Biology Division, San Francisco VA Health Care System, San Francisco, CA, 94121, USA. Gary.Cecchini@ucsf.edu.
Department of Biochemistry & Biophysics, University of California, San Francisco, CA, 94158, USA. Gary.Cecchini@ucsf.edu.

T M Iverson (TM)

Department of Pharmacology, Vanderbilt University, Nashville, TN, 37232, USA. tina.iverson@vanderbilt.edu.
Center for Structural Biology Vanderbilt University, Nashville, TN, 37232, USA. tina.iverson@vanderbilt.edu.
Department of Biochemistry, Vanderbilt University, Nashville, TN, 37232, USA. tina.iverson@vanderbilt.edu.
Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, TN, 37232, USA. tina.iverson@vanderbilt.edu.

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