Supramolecular Self-Assembly of Proteins Promoted by Hybrid Polyoxometalates.

hybrids metal-oxo clusters polyoxometalates proteins supramolecular assembly

Journal

Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338

Informations de publication

Date de publication:
11 Jan 2024
Historique:
received: 23 12 2023
medline: 12 1 2024
pubmed: 12 1 2024
entrez: 12 1 2024
Statut: aheadofprint

Résumé

Controlling the formation of supramolecular protein assemblies and endowing them with new properties that can lead to novel functional materials is an important but challenging task. In this work, a new hybrid polyoxometalate is designed to induce controlled intermolecular bridging between biotin-binding proteins. Such bridging interactions lead to the formation of supramolecular protein assemblies incorporating metal-oxo clusters that go from several nanometers in diameter up to the micron range. Insights into the self-assembly process and the nature of the resulting biohybrid materials are obtained by a combination of Small Angle X-ray Scattering (SAXS), Transmission Electron Microscopy (TEM), and Dynamic Light Scattering (DLS), along with fluorescence, UV-vis, and Circular Dichroism (CD) spectroscopy. The formation of hybrid supramolecular assemblies is determined to be driven by biotin binding to the protein and electrostatic interactions between the anionic metal-oxo cluster and the protein, both of which also influence the stability of the resulting assemblies. As a result, the rate of formation, size, and stability of the supramolecular assemblies can be tuned by controlling the electrostatic interactions between the cluster and the protein (e.g., through varying the ionic strength of the solution), thereby paving the way toward biomaterials with tunable assembly and disassembly properties.

Identifiants

pubmed: 38213017
doi: 10.1002/smll.202312009
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2312009

Subventions

Organisme : Research Foundation Flanders
Organisme : KU Leuven
Organisme : Onderzoeksraad, KU Leuven
ID : C14/19/076
Organisme : Vlaamse Overheid

Informations de copyright

© 2024 Wiley-VCH GmbH.

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Auteurs

David E Salazar Marcano (DE)

Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven, 3001, Belgium.

Sarah Lentink (S)

Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven, 3001, Belgium.

Jieh-Jang Chen (JJ)

Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven, 3001, Belgium.

Alexander V Anyushin (AV)

Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven, 3001, Belgium.

Mhamad Aly Moussawi (MA)

Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven, 3001, Belgium.

Jenna Bustos (J)

Department of Chemistry, Oregon State University, Corvallis, OR, 97331, USA.

Bart Van Meerbeek (B)

Department of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU Leuven, Kapucijnenvoer 7, Leuven, 3000, Belgium.

May Nyman (M)

Department of Chemistry, Oregon State University, Corvallis, OR, 97331, USA.

Tatjana N Parac-Vogt (TN)

Department of Chemistry, KU Leuven, Celestijnenlaan 200F, Leuven, 3001, Belgium.

Classifications MeSH