Comparison of telomere length in patients with bone marrow failure syndromes and healthy controls.
aplastic anemia
bone marrow failure
quantitative real-time polymerase chain reaction
telomere length
Journal
European journal of haematology
ISSN: 1600-0609
Titre abrégé: Eur J Haematol
Pays: England
ID NLM: 8703985
Informations de publication
Date de publication:
12 Jan 2024
12 Jan 2024
Historique:
revised:
02
01
2024
received:
21
09
2023
accepted:
03
01
2024
medline:
12
1
2024
pubmed:
12
1
2024
entrez:
12
1
2024
Statut:
aheadofprint
Résumé
During normal aging, telomeric DNA is gradually lost in dividing somatic cells, and critically short telomeres lead to replicative senescence, apoptosis, or chromosomal instability. We studied telomere length in bone marrow failure syndromes (BMFS) compared to normal healthy population. Peripheral blood was collected from the participants, and genomic DNA was extracted. Relative telomere length was measured using a quantitative polymerase chain reaction. Statistical analysis was performed using SPSS and GraphPad Prism 8.2 software. The median age of normal Indian population was 31 (0-60) years. As expected, telomere length (TL) showed a decline with age and no difference in TL between males and females. The median age of 650 patients with aplastic anemia (AA) was 30 (1-60) years. TL was significantly shorter in patients with AA compared to healthy controls (p < .001). In FA and MDS patients, TL was significantly shorter than age-matched healthy controls (p = .028; p < .001), respectively. There was no difference between the median TL in age-matched AA and FA patients (p = .727). However, patients with MDS had shorter TL than age-matched AA (p = .031). TL in BMF syndrome patients was significantly shorter than age-matched healthy controls.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Department of Biotechnology, Ministry of Science and Technology, India
ID : BT/PR17415/MED/12/724/2016
Informations de copyright
© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Références
Verdun RE, Karlseder J. Replication and protection of telomeres. Nature. 2007;447(7147):924-931. doi:10.1038/nature05976
Baird DM, Britt-Compton B, Rowson J, Amso NN, Gregory L, Kipling D. Telomere instability in the male germline. Hum Mol Genet. 2006;15(1):45-51. doi:10.1093/hmg/ddi424
Kong CM, Lee XW, Wang X. Telomere shortening in human diseases. FEBS J. 2013;280(14):3180-3193. doi:10.1111/febs.12326
Blackburn EH. Switching and signaling at the telomere. Cell. 2001;106(6):661-673. doi:10.1016/s0092-8674(01)00492-5
Wright WE, Shay JW. Telomere biology in aging and cancer. J Am Geriatr Soc. 2005;53:S292-S294. doi:10.1111/j.1532-5415.2005.53492.x
Maser RS, DePinho RA. Connecting chromosomes, crisis, and cancer. Science. 2002;297(5581):565-569. doi:10.1126/science.297.5581.565
Hiyama E, Hiyama K. Telomere and telomerase in stem cells. Br J Cancer. 2007;96:1020-1024. doi:10.1038/sj.bjc.6603671
Robert A, Brodsky RJJ. Aplastic anaemia. Lancet. 2005;365(1):1647-1656. doi:10.1179/1024533213Z.000000000233
Young NS, Calado RT, Scheinberg P. Current concepts in the pathophysiology and treatment of aplastic anemia. Blood. 2006;108(8):2509-2519. doi:10.1182/blood-2006-03-010777
Young NS. Acquired aplastic anemia. Ann Intern Med. 2002;136(7):534-546. doi:10.7326/0003-4819-136-7-200204020-00011
Ball BSE, Gibson FM, Tooze JA, Marsh JCW, Gordon-smith EC. Progressive telomere shortening in aplastic anemia. Blood. 1998;91(10):3582-3592.
Brümmendorf TH, Maciejewski JP, Mak J, Young NS, Lansdorp PM. Telomere length in leukocyte subpopulations of patients with aplastic anemia. Blood. 2001;97(4):895-900. doi:10.1182/blood.V97.4.895
Scheinberg P, Cooper JN, Sloand EM, Wu CO, Calado RT, Young NS. Association of telomere length of peripheral blood leukocytes with hematopoietic relapse, malignant transformation, and survival in severe aplastic anemia. JAMA. 2010;304(12):1358-1364. doi:10.1001/jama.2010.1376
Shahinaz MG, Wang T, Haagenson M, et al. Immaculata De Vivo and SAS. Association between donor leukocyte telomere length and survival after unrelated allogeneic hematopoietic cell transplantation for severe aplastic anemia. JAMA. 2015;313(6):594-602. doi:10.1001/jama.2015.7
Wang Y, McReynolds LJ, Dagnall C, et al. Pre-transplant short telomeres are associated with high mortality risk after unrelated donor haematopoietic cell transplant for severe aplastic anaemia. Br J Haematol. 2020;188(2):309-316. doi:10.1111/bjh.16153
Adhikari S, Nayek K, Bandyopadhyay A, Mandal P. Implication of therapeutic outcomes associated with molecular characterization of paediatric aplastic anaemia. Biochem Biophys Rep. 2021;25:100899. doi:10.1016/j.bbrep.2020.100899
Mandal P, Dutta S. Landscape of granulocyte telomere shortening in cytopenia in India. Res Sq. 2020. doi:10.21203/rs.3.rs-36887/v1
Fiesco-Roa MO, Giri N, McReynolds LJ, Best AF, Alter BP. Genotype-phenotype associations in Fanconi anemia: a literature review. Blood Rev. 2019;37:37. doi:10.1016/j.blre.2019.100589
Leteurtre F, Li X, Guardiola P, et al. Accelerated telomere shortening and telomerase activation in Fanconi's anaemia. Br J Haematol. 1999;105(4):883-893. doi:10.1046/j.1365-2141.1999.01445.x
Hanson H, Mathew CG, Docherty Z, Mackie OC. Telomere shortening in Fanconi anaemia demonstrated by a direct FISH approach. Cytogenet Cell Genet. 2001;93(3-4):203-206. doi:10.1159/000056985
Fenaux P, Platzbecker U, Ades L. How we manage adults with myelodysplastic syndrome. Br J Haematol. 2020;189(6):1016-1027. doi:10.1111/bjh.16206
Lange K, Holm L, Nielsen KV, et al. Telomere shortening and chromosomal instability in myelodysplastic syndromes. Genes Chromosomes Cancer. 2010;49(3):260-269. doi:10.1002/GCC.20737
Göhring G, Lange K, Hofmann W, et al. Telomere shortening, clonal evolution and disease progression in myelodysplastic syndrome patients with 5q deletion treated with lenalidomide. Leukemia. 2012;26(2):356-358. doi:10.1038/leu.2011.193
Rollison DE, Epling-Burnette PK, Park JY, et al. Telomere length in myelodysplastic syndromes. Leuk Lymphoma. 2011;52(8):1528-1536. doi:10.3109/10428194.2011.568648
Scheinberg P, Wu CO, Nunez O, Young NS. Predicting response to immunosuppressive therapy and survival in severe aplastic anaemia. Br J Haematol. 2009;144(2):206-216. doi:10.1111/j.1365-2141.2008.07450.x
Greenberg PL, Tuechler H, Schanz J, et al. Revised international prognostic scoring system for myelodysplastic syndromes. Blood. 2012;120(12):2454-2465. doi:10.1182/BLOOD-2012-03-420489
Cawthon RM. Telomere measurement by quantitative PCR. Nucleic Acids Res. 2002;30(10):e47. doi:10.1093/nar/30.10.e47
Barade A, Aboobacker F, Korula A, et al. Impact of donor telomere length on survival in patients undergoing matched sibling donor transplantation for aplastic anaemia. Br J Haematol. 2022;196(3):724-734. doi:10.1111/bjh.17880
de Meyer T, Nawrot T, Bekaert S, de Buyzere ML, Rietzschel ER, Andrés V. Telomere length as cardiovascular aging biomarker: JACC review topic of the week. J Am Coll Cardiol. 2018;72(7):805-813. doi:10.1016/J.JACC.2018.06.014
Factor-Litvak P, Susser E, Kezios K, et al. Leukocyte telomere length in newborns: implications for the role of telomeres in human disease. Pediatrics. 2016;137(4):e20153927. doi:10.1542/peds.2015-3927
Helby J, Nordestgaard BG, Benfield T, Bojesen SE. Shorter leukocyte telomere length is associated with higher risk of infections: a prospective study of 75,309 individuals from the general population. Haematologica. 2017;102(8):1457-1465. doi:10.3324/haematol.2016.161943
Nguyen MT, Lycett K, Vryer R, et al. Telomere length: population epidemiology and concordance in Australian children aged 11-12 years and their parents. BMJ Open. 2019;9:118-126. doi:10.1136/bmjopen-2017-020263
Bhupatiraju C, Saini D, Patkar S, Deepak P, Das B, Padma T. Association of shorter telomere length with essential hypertension in Indian population. Am J Hum Biol. 2012;24(4):573-578. doi:10.1002/ajhb.22264
Yadav S, Maurya PK. Correlation between telomere length and biomarkers of oxidative stress in human aging. Rejuvenation Res. 2022;25(1):25-29. doi:10.1089/rej.2021.0045
Savage SA, Alter BP. The role of telomere biology in bone marrow failure and other disorders. Mech Ageing Dev. 2008;129(1-2):35-47. doi:10.1016/j.mad.2007.11.002
Song JY, Kuang LP, Wang Y, et al. The relationship of telomere and telomerase activity with outcome of aplastic anemia after immunosuppressive therapy. Zhonghua Xue Ye Xue Za Zhi. 2013;34(9):771-776. doi:10.3760/cma.j.issn.0253-2727.2013.09.008
Alter BP, Giri N, Savage SA, Rosenberg PS. Telomere length in inherited bone marrow failure syndromes. Haematologica. 2015;100(1):49-54. doi:10.3324/haematol.2014.114389
Miwata S, Narita A, Okuno Y, et al. Clinical diagnostic value of telomere length measurement in inherited bone marrow failure syndromes. Haematologica. 2021;106(9):2511-2515. doi:10.3324/haematol.2021.278334
Myllymäki M, Redd R, Reilly CR, et al. Short telomere length predicts nonrelapse mortality after stem cell transplantation for myelodysplastic syndrome. Blood. 2020;136(26):3070-3081. doi:10.1182/blood.2020005397
Hwang SM, Kim SY, Kim JA, et al. Short telomere length and its correlation with gene mutations in myelodysplastic syndrome. J Hematol Oncol. 2016;9(1):62. doi:10.1186/s13045-016-0287-9
Shammas MA. Telomeres, lifestyle, cancer, and aging. Curr Opin Clin Nutr Metab Care. 2011;14(1):28-34. doi:10.1097/MCO.0B013E32834121B1
Yamaguchi H, Calado RT, Ly H, et al. Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia. N Engl J Med. 2005;352(14):1413-1424. doi:10.1056/NEJMoa042980
Khincha PP, Dagnall CL, Hicks B, et al. Correlation of leukocyte telomere length measurement methods in patients with dyskeratosis congenita and in their unaffected relatives. Int J Mol Sci. 2017;18(8):1-8. doi:10.3390/ijms18081765
Gutierrez-Rodrigues F, Santana-Lemos BA, Scheucher PS, Alves-Paiva RM, Calado RT. Direct comparison of flow-FISH and qPCR as diagnostic tests for telomere length measurement in humans. PLoS One. 2014;9(11):1-9. doi:10.1371/journal.pone.0113747