Mathematical modeling of fluid dynamics in in vitro gut fermentation systems: A new tool to improve the interpretation of microbial metabolism.
accumulation
bacteria
digestion
dilution
gut microbiota
metabolism
metabolites
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
31 Jan 2024
31 Jan 2024
Historique:
revised:
13
12
2023
received:
28
08
2023
accepted:
15
12
2023
medline:
12
1
2024
pubmed:
12
1
2024
entrez:
12
1
2024
Statut:
ppublish
Résumé
In vitro systems are widely employed to assess the impact of dietary compounds on the gut microbiota and their conversion into beneficial bacterial metabolites. However, the complex fluid dynamics and multi-segmented nature of these systems can complicate the comprehensive analysis of dietary compound fate, potentially confounding physical dilution or washout with microbial catabolism. In this study, we developed fluid dynamics models based on sets of ordinary differential equations to simulate the behavior of an inert compound within two commonly used in vitro systems: the continuous two-stage PolyFermS system and the semi-continuous multi-segmented SHIME® system as well as into various declinations of those systems. The models were validated by investigating the fate of blue dextran, demonstrating excellent agreement between experimental and modeling data (with r
Identifiants
pubmed: 38214938
doi: 10.1096/fj.202301739RR
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23398Subventions
Organisme : NSERC-Diana Food Industrial Chair on prebiotic effects of fruits and vegetables
Organisme : NSERC funded Collaborative Research and Development program on the development of prebiotic polyphenols and probiotic bacteria
Organisme : Canada Research Excellence Chair in the Microbiome-Endocannabinoidome Axis in Metabolic Health
Organisme : NSERC
Informations de copyright
© 2024 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
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