Sensitive simultaneous measurements of oxygenation and extracellular pH by EPR using a stable monophosphonated trityl radical and lithium phthalocyanine.
EPR
ESR
Hypoxia
In vivo
Metabolism
Mitochondria
Oxygenation
Tumor microenvironment
pH
Journal
Free radical biology & medicine
ISSN: 1873-4596
Titre abrégé: Free Radic Biol Med
Pays: United States
ID NLM: 8709159
Informations de publication
Date de publication:
11 Jan 2024
11 Jan 2024
Historique:
received:
20
11
2023
revised:
09
01
2024
accepted:
10
01
2024
medline:
14
1
2024
pubmed:
14
1
2024
entrez:
13
1
2024
Statut:
aheadofprint
Résumé
The monitoring of acidosis and hypoxia is crucial because both factors promote cancer progression and impact the efficacy of anti-cancer treatments. A phosphonated tetrathiatriarylmethyl (pTAM) has been previously described to monitor both parameters simultaneously, but the sensitivity to tackle subtle changes in oxygenation was limited. Here, we describe an innovative approach combining the pTAM radical and lithium phthalocyanine (LiPc) crystals to provide sensitive simultaneous measurements of extracellular pH (pH
Identifiants
pubmed: 38218552
pii: S0891-5849(24)00012-1
doi: 10.1016/j.freeradbiomed.2024.01.012
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2024. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflict of interest.