Method for taking Scanning Electron Microscope photographs of nematodes and meiofauna with the support of a low-cost and easy-made container.

SEM critical point drying dehydration hand-made tool

Journal

Helminthologia
ISSN: 0440-6605
Titre abrégé: Helminthologia
Pays: Germany
ID NLM: 0043764

Informations de publication

Date de publication:
Dec 2023
Historique:
received: 07 06 2023
accepted: 06 10 2023
medline: 15 1 2024
pubmed: 15 1 2024
entrez: 15 1 2024
Statut: epublish

Résumé

This paper presents a method for capturing Scanning Electron Microscope (SEM) photographs of small specimens, including nematodes, arthropods, small insects, and other meiofauna. Our method is tailored to handle nematode specimens mounted on permanent slides, an area with relatively limited documentation. Besides, the process of transferring such delicate specimens from one solution to another has historically posed numerous challenges. To address this issue, we introduce a low-cost and easy-made container designed specifically to facilitate the aforementioned procedure, with a particular focus on SEM photography. The newly introduced container offers a practical solution that enhances the efficiency and effectiveness of specimen handling, ultimately enabling high-quality SEM imaging. This method holds significant promise for researchers working in the field of micro-scopic organism analysis, providing a valuable tool for their investigations with minimum cost.

Identifiants

pubmed: 38222489
doi: 10.2478/helm-2023-0041
pii: helm-2023-0041
pmc: PMC10787630
doi:

Types de publication

Journal Article

Langues

eng

Pagination

393-396

Informations de copyright

© 2023 H. T. Nguyen et al., published by Sciendo.

Déclaration de conflit d'intérêts

Conflict of Interest Authors state no conflict of interest.

Auteurs

H T Nguyen (HT)

Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.

T D Nguyen (TD)

Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.

T M L Le (TML)

Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.

Q P Trinh (QP)

Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.

Classifications MeSH