Method for taking Scanning Electron Microscope photographs of nematodes and meiofauna with the support of a low-cost and easy-made container.
SEM
critical point drying
dehydration
hand-made tool
Journal
Helminthologia
ISSN: 0440-6605
Titre abrégé: Helminthologia
Pays: Germany
ID NLM: 0043764
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
07
06
2023
accepted:
06
10
2023
medline:
15
1
2024
pubmed:
15
1
2024
entrez:
15
1
2024
Statut:
epublish
Résumé
This paper presents a method for capturing Scanning Electron Microscope (SEM) photographs of small specimens, including nematodes, arthropods, small insects, and other meiofauna. Our method is tailored to handle nematode specimens mounted on permanent slides, an area with relatively limited documentation. Besides, the process of transferring such delicate specimens from one solution to another has historically posed numerous challenges. To address this issue, we introduce a low-cost and easy-made container designed specifically to facilitate the aforementioned procedure, with a particular focus on SEM photography. The newly introduced container offers a practical solution that enhances the efficiency and effectiveness of specimen handling, ultimately enabling high-quality SEM imaging. This method holds significant promise for researchers working in the field of micro-scopic organism analysis, providing a valuable tool for their investigations with minimum cost.
Identifiants
pubmed: 38222489
doi: 10.2478/helm-2023-0041
pii: helm-2023-0041
pmc: PMC10787630
doi:
Types de publication
Journal Article
Langues
eng
Pagination
393-396Informations de copyright
© 2023 H. T. Nguyen et al., published by Sciendo.
Déclaration de conflit d'intérêts
Conflict of Interest Authors state no conflict of interest.