Ovine model of congenital chest wall and spine deformity: From birth to 3 months follow-up.

Background

The evolution and treatment of lung alterations related to congenital spine and chest wall deformities (CWD) are poorly understood. Most animal models of CWD created postnatally were not evaluated for respiratory function. The goal of our study was to evaluate the effects of a CWD induced in utero on lung growth and function in an ovine model.

Methods

A CWD was induced in utero at 70-75 days of gestation in 14 ovine fetuses by resection of the 7th and 8th left ribs. Each non-operated twin fetus was taken as control. Respiratory mechanics was studied postnatally in the first week and at 1, 2, and 3 months. Post-mortem respiratory mechanics and lung histomorphometry were also assessed at 3 months.

Results

Eight out of 14 CWD lambs (57%) and 14 control lambs survived the postnatal period. One severe and five mild deformities were induced. At birth, inspiratory capacity (25 vs. 32 mL/kg in controls), and dynamic (1.4 vs. 1.8 mL/cmH

Conclusions

Our study is the first to assess the effects of a prenatally induced CWD on lung development and function from birth to 3 months in an ovine model. Our results show no significant differences in lung histomorphometry at 3 months in CWD lambs compared to controls. Resolution at 1 month of the alterations in respiratory mechanics present at birth may be related to the challenge in inducing severe deformities.

Informations de copyright

© 2023 The Authors. JOR Spine published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.

Déclaration de conflit d'intérêts

Jesse Shen has received traveling fellowship support from the Scoliosis Research Society. Nathalie Samson, Jérôme Lamontagne‐Proulx, Denis Soulet, Yves Tremblay, Charlène Nadeau, Sarah Bouchard, Marc Bazin, Jean‐Paul Praud declare that they have no conflict of interest. Stefan Parent has received royalties from EOS imaging, he is Co‐founder of the company Spinologics Inc., he received consultancy fees from K2M, Medtronic, and DePuy Synthes Spine, he received grants from DePuy Synthes Spine, Canadian Institutes of Health Research, Pediatric Orthopaedic Society of North America, Scoliosis Research Society, Medtronic, EOS imaging, Canadian Foundation for Innovation, Setting Scoliosis Straight Foundation, Natural Sciences and Engineering Council of Canada, Fonds de recherche Québec ‐ Santé, grants and Orthopaedic Research and Education Foundation, he received fellowship support from DePuy Synthes and Medtronic, he is the holder of the Academic Chair in Pediatric Spinal Deformities of CHU Ste‐Justine, he is member of speaker bureau of Orthopaediatrics.