Pooled screening with next-generation gene editing tools.

Pooled screening creates a pool of cells with genetic variants, allowing for the simultaneous examination for changes in behavior or function. By selectively inducing mutations or perturbing expression, it enables scientists to systematically investigate the function of genes or genetic elements. Emerging gene editing tools, such as CRISPR, coupled with advances in sequencing and computational capabilities, provide growing opportunities to understand biological processes in humans, animals, and plants as well as to identify potential targets for therapeutic interventions and agricultural research. In this review, we highlight the recent advances of pooled screens using next-generation gene editing tools along with relevant challenges and describe potential future directions of this technology.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: SC is a (co)founder of EvolveImmune Tx, Cellinfinity Bio, Chen Consulting, NumericGlobal, and Chen Tech, all unrelated to this study.