Circle-seq based method for eccDNA synthesis and its application as a canonical promoter independent vector for robust microRNA overexpression.
Artificial eccDNA
CAES
Circle-seq
DdPCR
EccDNA
EccDNA function
MicroRNA therapeutics
Journal
Computational and structural biotechnology journal
ISSN: 2001-0370
Titre abrégé: Comput Struct Biotechnol J
Pays: Netherlands
ID NLM: 101585369
Informations de publication
Date de publication:
Dec 2024
Dec 2024
Historique:
received:
11
10
2023
revised:
05
12
2023
accepted:
15
12
2023
medline:
15
1
2024
pubmed:
15
1
2024
entrez:
15
1
2024
Statut:
epublish
Résumé
Extrachromosomal circular DNA (eccDNA) has recently gained increasing attention due to its significant role in cancer and other pathophysiologic states. The majority of circular DNAs detected by Circle-seq are small-size eccDNAs with enigmatic functions. One major technical hurdle is to synthesize eccDNA for functional identification. Here, we describe CAES (
Identifiants
pubmed: 38223344
doi: 10.1016/j.csbj.2023.12.019
pii: S2001-0370(23)00491-9
pmc: PMC10788182
doi:
Types de publication
Journal Article
Langues
eng
Pagination
358-368Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.