Hemolymph exosomes inhibit Spiroplasma eriocheiris infection by promoting Tetraspanin-mediated hemocyte phagocytosis in crab.
crab
exosome
hemocyte phagocytosis
intracellular pathogen
tetraspanin
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
31 Jan 2024
31 Jan 2024
Historique:
revised:
26
12
2023
received:
25
10
2023
accepted:
04
01
2024
medline:
16
1
2024
pubmed:
16
1
2024
entrez:
16
1
2024
Statut:
ppublish
Résumé
Exosomes released from infected cells are thought to play an important role in the dissemination of pathogens, as well as in host-derived immune molecules during infection. As an intracellular pathogen, Spiroplasma eriocheiris is harmful to multiple crustaceans. However, the immune mechanism of exosomes during Spiroplasma infection has not been investigated. Here, we found exosomes derived from S. eriocheiris-infected crabs could facilitate phagocytosis and apoptosis of hemocytes, resulting in increased crab survival and suppression of Spiroplasma intracellular replication. Proteomic analysis revealed the altered abundance of EsTetraspanin may confer resistance to S. eriocheiris, possibly by mediating hemocyte phagocytosis in Eriocheir sinensis. Specifically, knockdown of EsTetraspanin in E. sinensis increased susceptibility to S. eriocheiris infection and displayed compromised phagocytic ability, whereas overexpression of EsTetraspanin in Drosophila S2 cells inhibited S. eriocheiris infection. Further, it was confirmed that intramuscular injection of recombinant LEL domain of EsTetraspanin reduced the mortality of S. eriocheiris-infected crabs. Blockade with anti-EsTetraspanin serum could exacerbate S. eriocheiris invasion of hemocytes and impair hemocyte phagocytic activity. Taken together, our findings prove for the first time that exosomes modulate phagocytosis to resist pathogenic infection in invertebrates, which is proposed to be mediated by exosomal Tetraspanin, supporting the development of preventative strategies against Spiroplasma infection.
Identifiants
pubmed: 38226893
doi: 10.1096/fj.202302182R
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23433Subventions
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 31870168
Organisme : MOST | National Natural Science Foundation of China (NSFC)
ID : 31570176
Organisme : the "JBGS" Project of Seed Industry Revitalization in Jiangsu Province
ID : JBGS [2021]031
Organisme : Jiangsu Agricultural Science and Technology Innovation Fund (JASTIF)
ID : CX (21)3157
Organisme : Modern Agricultural Industry Technology System Project of Jiangsu Province
ID : JATS [2022]344
Informations de copyright
© 2024 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
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