An octameric PqiC toroid stabilises the outer-membrane interaction of the PqiABC transport system.
Envelope Spanning
Gram Negative Cell Envelope
Lipid Transport
Outer Membrane Biogenesis
Pqi
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
16 Jan 2024
16 Jan 2024
Historique:
received:
02
08
2023
accepted:
10
11
2023
revised:
31
10
2023
medline:
17
1
2024
pubmed:
17
1
2024
entrez:
16
1
2024
Statut:
aheadofprint
Résumé
The E. coli Paraquat Inducible (Pqi) Pathway is a putative Gram-negative phospholipid transport system. The pathway comprises three components: an integral inner membrane protein (PqiA), a periplasmic spanning MCE family protein (PqiB) and an outer membrane lipoprotein (PqiC). Interactions between all complex components, including stoichiometry, remain uncharacterised; nevertheless, once assembled into their quaternary complex, the trio of Pqi proteins are anticipated to provide a continuous channel between the inner and outer membranes of diderms. Here, we present X-ray structures of both the native and a truncated, soluble construct of the PqiC lipoprotein, providing insight into its biological assembly, and utilise neutron reflectometry to characterise the nature of the PqiB-PqiC-membrane interaction. Finally, we employ phenotypic complementation assays to probe specific PqiC residues, which imply the interaction between PqiB and PqiC is less intimate than previously anticipated.
Identifiants
pubmed: 38228789
doi: 10.1038/s44319-023-00014-4
pii: 10.1038/s44319-023-00014-4
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : UKRI | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/M01116X/1
Organisme : UKRI | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/S017283/1
Organisme : UKRI | STFC | ISIS Neutron and Muon Source (The ISIS Neutron and Muon Source)
ID : RB2300004
Organisme : UKRI | STFC | ISIS Neutron and Muon Source (The ISIS Neutron and Muon Source)
ID : RB2220703
Organisme : Diamond Light Source
ID : mx26803
Informations de copyright
© 2024. The Author(s).
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